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THB/SO2-2 Operator’s Manual – OPTI CCA-TS
tHb/SO
2
ANALYTES
Measurement Principle
The measurement of total Hemoglobin ( c tHb) and oxygen saturation ( S O
2
)
uses the well-established
principle of optical reflectance. Red and infrared light at three wavelengths is directed at whole, non-
hemolyzed blood within a precisely-defined part of the cassette over the O
2
optode. The photons are
partially absorbed and reflected by erythrocytes in a manner proportional to hemoglobin level; at low
hemoglobin levels the unabsorbed photons strike the O
2
optode’s pink overcoat and are reflected back
up through the blood a second time. A portion of the reflected light exits the top of the cassette and is
measured by a detector in the instrument. The infrared wavelengths are selected for the hemoglobin
measurement because they are largely independent of S O
2
, that is, the predominate forms of adult and
fetal hemoglobin absorb similarly within the 750-850 nm wavelength range. The red wavelength is
utilized for the
S
O
2
measurement because it is much more strongly absorbed by deoxyhemoglobin than
all other hemoglobins, and it is picked close to the isosbestic point for oxy- and carboxyhemoglobin.
Sensitivity to erythrocyte aggregation (rouleaux formation) is minimized by maintaining high shear force
just prior to measurement (see Interferences below).
Measurement Range
Range Resolution (Low/High) Units
tHb 5 to 25 0.1 g/dL
S O
2
60 to 100 1/0.1 %
Standard Reference Cassette (SRC) Limit Values
LOW NORMAL HIGH Units
tHb 20.0 ± 1.5 14.0 ± 1.5 8.0 ± 1.5 g/dL
SO
2
70.0 ± 2 90.0 ± 2 98.0 ± 2 %
Interferences
The following interferents were quantified in whole blood, showing sensitivity to dyes similar to most
CO-oximeters:
Substance amount ctHb change (g/dL) SO
2
change (%)
EXOGENOUS
Cardio (Indocyanine) Green 0.5 mg/dL +4.7 +4%
Evan’s Blue 5.0 mg/dL < 1 -17%
Methylene Blue 25 mg/dL +3.0 -37%
ENDOGENOUS
Carboxyhemoglobin 10% -2.0 < 2%
Carboxyhemoglobin 20% -3.3 < 2%
Methemoglobin 13% +1.9 -7%