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Operator’s Manual – OPTI CCA-TS Lac-1
ANALYTES
LACTATE
Lactate (B-Lac Cassette)
Clinical Significance
1
Lactic Acid is produced as an intermediate in carbohydrate metabolism. The blood lactate concentration
is primarily related to the rate of lactate production in white skeletal muscle, the brain, renal medulla and
erythrocytes and the rate of lactate metabolism of the liver and kidneys. High lactate levels, coupled with
a pH of less than 7.25 may indicate Lactic Acidosis.
Lactic Acidosis has two clinically significant types: 1) hypoxic which is associated with lowered
availability of oxygen to the body tissues and 2) metabolic which is associated with disease, drugs/toxins
or inborn metabolic issues.
Hypoxia is the most common cause of the lactic acidosis and may indicate sepsis, shock, hypovolemia,
hypo-perfusion and left ventricular failure. Types of hypoxia include:
• Anemic Hypoxia : Hypoxia due to lowered oxygen-carrying capacity of the blood; this may be
either from a decrease in total hemoglobin or a change in components of the hemoglobin.
• Stagnant Hypoxia : A type seen when not enough oxygen is transported by the blood because
blood flow is reduced, such as with heart failure.
• Histotoxic Hypoxia : Hypoxia that is due to impaired use of oxygen by tissues
• Hypoxic Hypoxia : Hypoxia that is due to insufficient oxygen reaching the blood.
• Ischemic Hypoxia : Hypoxia that occurs when blood flow to tissue is low.
Measurement Principle
1
The OPTI Medical lactate biosensor contains the enzyme lactate oxidase to selectively catalyze the
reaction between lactate and oxygen, as outlined in the reaction sequence.
L-L O
2
(Lactate Oxidase) Py H
2
O
2
The oxygen consumption is measured photochemically by an optical sensor. The rate of oxygen
consumption is proportional to the concentration of lactate in the specimen.
Measurement Range
Range Resolution (Low/High) Units
0.3 – 17.5 0.01 / 0.01 mmol/L
2.7 – 157.7 0.1 / 0.1 mg/dL