47
Gentier Real-Time PCR System
V.
Melting
: melting stage allows the system to read the fluorescence signals after each
temperature increment. If the current step is the last step of melting stage, user can set the
temperature
Increment
in the
Step Setting
dialog box for the current step, as shown in
figure 5.6f.
Figure D-5.6f Step Setting Dialog Box – Meltingt Step
▶
Increment
: the temperature change(°C) after which the system will read the fluorescence.
User can use
and
keys, or manually enter the temperature increment in the
Increment
input box.
Reminding
: the temperature increment setting range is 0.1°C 5.0°C.
VI.
Continuous Melting
: continuous melting stage allows the system to read the fluorescence
more frequently. If the current step is the last step of continuous melting stage, user can set
the fluorescence reading times per °C for the current step in the
Step Setting
dialog box, as
shown in figure 5.6g.
Figure D-5.6g Step Setting Dialog Box – Continuous Meltingt Step Mode
▶
Readings
: the fluorescence reading times per °C. User can use
and
keys, or
manually enter the fluorescence reading frequence in the
Readings
input box.
Reminding
: the fluorescence reading frequence setting range is 2 readings/°C – 15
readings/°C.