42
Gentier Real-Time PCR System
Introduction to Stage Type Window
▶
The application software provides the following stage types:
Preincubation
,
Reverse
Transcription
,
2 Step Amplification
,
3 Step Amplification
,
Melting
,
Continuous Melting
and
Cooling
. User can choose the corresponding predefined stage in the stage type window
and click
<Add >
to add the selected stage; or double click any stage to add directly.
▶
User could select
Custom Stage
and click
< Add >
to define the stage setting according to
specific experiment requirements.
▶
After adding a stage, please click
< Close >
to return to the run setting interface. The new
stage will be added to the temperature program area and stage list.
Reminding
: at least one stage should be included in the temperature program.
4. Step list
: as shown in figure D-5.5.
Figure D 5.5 Step list
Step List Introduction
▶
▶
▶
▶
▶
Step
: display the number of each step;
Temperature
: display the target temperature of the current step.;
Time
: display the temperature holding time of the current step;
Fluorescence
:
display whether to read fluorescence at the current step;
User can add/delete the step according to the experiment requirements or adjust the
sequence of each step.
User can click
<
Add >
icon to add a new temperature step.
User can click
<
Delete >
icon to delete the selected step.
