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Gentier Real-Time PCR System
Figure D- 15. High Resolution Melting Analysis Setting interface - High Resolution Melting tab
▶
Normalization Method
:
the application software provides two normalization methods:
Ratio Method
and
Exponential Method.
The
Ratio Method
option is selected by default.
▶
Fluorescence Normalization
:
used to set the temperature range for the fluorescence
normalization of the melting curve.
a.
Pre-Melt Range
: user can use
and
keys to set the pre-melting temperature range
of the normalization area of the melting curve.
b.
Post-Melt Range
: user can use
and
keys to set the post-melting temperature
range of the normalization area ofthe melting curve.
▶
Sensitivity:
used to set the discrimination sensitivity of melting curve.
Reminding
: by default, the application software will automatically set the corresponding
parameters for the fluorescence normalization of melting curve; user can also drag the
relevant threshold lines (vertical sliders) on the
Melting Curve
functioncal module to
determine the normalized area of melting curve.
