82
Gentier Real-Time PCR System
Interface Introduction and Parameter Description
Reminding
: the parameter descriptions of
Sample Setting, Result Table
and
Heat Map
functional modules are same with those introduced in
D-3.3.4.1 Abs Quant
.
I.
Melting Curve
: display the fluorescence intensity against the temperature, as shown in
figure D-14.1.
Figure D 14.1 HRM analysis interface Melting Curve functional module
▶
Parameter introduction
:
X axis represents the temperature;
Y axis represents the Relative Fluorescence Unit (RFU);
By default, the application software will automatically determine normalized area of
melting curve. The two vertical sliders on the left are the
Pre-melting Range
threshold lines,
which are used to specify the pre-melting temperature range; while two vertical sliders on
the right are the
Post-melting Range
threshold lines, which are used to specify the
post-melting temperature range; the single line at the bottom is the
Pos/Neg Threshold
line,
which is used to determine the positivity or negativity of sample.
▶
Function key introduction
:
The introduction of
<
Y-axis Coordinate Adjustment >
and
<
Export >
keys are
same with those keys introduced in
D-3.3.4.1 Abs Quant - Amplification Curve
.
User can manually drag the vertical sliders or enter relevant parameter in the high
resolution melting analysis setting window to specify the normalized area of melting curve.
Reminding
: if the normalized area is manually changed, the relevant curve shape on
