80
Gentier Real-Time PCR System
User can click the
<
Analysis Setting >
icon in Tool Bar and the application software will
automatically pop up the melting curve analysis setting window, which consists of
Dye and
Gene, Sample
and
PeakArea
three tabs. User can set the relevant parameters for melting curve
analytical method in the melting curve analysis setting window.
I.
Melt Curve
tab: display the parameter range of each peak area, as shown in figure D-13.3.
Figure D-13.3 Melting Curve Analysis Setting interface - Melt Curve tab
Dye
: display the settings of
Dye
and its marked
Gene
of the current experiment, as shown in
figure D-13.3.
PeakArea
: display the melting peak area appearing with temperature rise.
User can use
and
keys to set the temperature range of melting peak area in
Min
Temperature
and
Max Temperature
columns.
User can use
and
keys to set the fluorescence range of melting area in
Min -df/dT
and
Max -df/dT
columns.
II.
Gene and Sample
tab: the introduction of relevant parameters and function keys is same
with those introduced in
D-3.3.4.1Abs Quant - Abs QuantAnalysis Setting.
