45
Gentier Real-Time PCR System
Reminding
: the initial and target temperature setting range is 0.0 °C - 100.0 °C.
Reminding
: the start cycle setting range is 1-max cycle number ofthe current stage.
Reminding
: the Delta Temperature setting range is 0.1°C- 5.0°C.
III.
Long Step Mode
: the long step mode allows the temperature program to change the
elongation step temperature holding time from the initial time to the target time as the
cycling proceeds. User could set the corresponding parameters in the
Step Setting
dialog
box, as shown in figure 5.6c.
Figure D-5.6c Step Setting Dialog Box – Long Step Mode
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IV.
Initial Time
: the initial value of the elongation time change range.
Target Time
: the target value ofthe elongation time change range.
Delta Time
: the time change per cycle.
Start Cycle
: the cycle number after which the time change is started.
Reminding
: the initial initial and target time setting range is 1s~60min.
Reminding
: the delta time setting range is 1s~10min.
Reminding
: the star cycle range is 1~ max cycle number ofthe current stage.
Gradient Step Mode
: the gradient step mode allows the sample block to adopt different
temperatures. User can set the
Temperature Center
and
Temperature Ofset
values for
the current gradient step, as shown in figure 5.6d. The system will then automatically
calculate the corespinding gradient temperatures.
