Drawell Gentier 96C, Руководство пользователя

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Gentier Real-Time PCR System
▶
Analysis Mode
: user can check the analysis mode check box of
Reference Dye
and
Baseline
Gain Calibration
according to the experiment requirements, and set the corresponding
parameters in the
Baseline
list.
a. Baseline Gain Calibration
: in general conditions, the application software select the
Baseline Gain Calibration
analysis mode by default, which provides user with
Automatic
Baseline
and
Manual Baseline
two baseline setting methods.
User can click the
Automatic Baseline
option, the application software will automatically
set the baseline for amplification curve, and shown the
Start Cycle
and
End Cycle
of the
automatic baseline setting in the coloumns of the
Baseline
list.
User can also click the
Manual Baseline
option, select one well in the
Baseline
list, use
and keys to maunually set the
Start Cycle
and
End Cycle
of the baseline setting for this
sample; or choose multiple wells and set the
Start Cycle
and
End Cycle
for
All Selected
Rows
on top of the
Baseline
list.
User can click
< Restore >
on the top right corner of
Baseline
list to restore the
Start Cycle
and
End Cycle
ofthe automatic baseline setting.
b. Reference Dye
: If user had set the reference dye in the Sample Setting tab according to the
experiment requirements, user can check the analysis mode check box of
Reference Dye
e
to analysis experiment data.
▶
Analytical Method
: the application software provides user with two Ct value analytical
methods:
Auto Threshold
,
Manual Threshold
and
Normalization Method
.
a. User can click
Auto Threshold
option, the application software will automatically set and
show the threshold value for all dyes in its colounm.
b. User can also click
Manual Threshold
option, select one fluorescein and use and
keys to set the manual threshold value in its colounm.
c. User can click
< Restore >
on the top right corner of threshold list to restore the
Auto
Threshold
settings.
d. User can click
Normalization Method
option and the application software will
automatically calculate the Ct value according to the normalization value of amplification
curve.