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Gentier Real-Time PCR System
temperature holding time from the initial time to the target time as the cycling proceeds.
▶
User can press the input boxes of
Initial Time
,
Target Time
,
Delta Time
and
Start Cycle
to edit the corresponding parameters for the current long step, as shown in figure E-3.6a.
4)
Figure E-3.6a Step Edit window - Long step parameter setting
Initial Time
: the initial value of the elongation time change range.
Target Time
: the target value of the elongation time change range.
Delta Time
: the temperature change(°C) per cycle.
Start Cycle
: the cycle number after which the time change is started.
Reminding
: the initial and target time setting range is 1s~60min.
Reminding
: the delta time setting range is 1s~10min.
Reminding
: the star cycle range is 1~ max cycle number ofthe current stage.
Reminding
: the other parameter settings are same with the standard step mode.
If the
Gradient
step mode option is selected, the step edit window will be shown as figure
E-3.7.
