101
Gentier Real-Time PCR System
Temperature Program Setting Operation
Step 1
:
Add stage
User can press
<
Add >
in the stage setting box and the instrument software will
automatically pop up a stage type selection window, as shown in figure E-3.2.
Figure E 3.2 Temperature Setting sub tab Stage type selection window
▶
▶
▶
Seven predefined stage types are provided by the instrument software for user:
Preincubation
,
Reverse Transcription, 2 Step Amplification
,
3 Step Amplification
,
Melting
,
Continuous Melting
and
Cooling
. User can also press
Custom Stage
to define the
stage setting according to specific experiment requirements.
User can press
< Add >
to add the selected stage to stage setting box.
User can press
< Back >
to return back to the run setting interface.
Reminding
: at least one stage should be included in the temperature program.
Step 2
:
Edit stage
▶
▶
The added stage will be automatically displayed in the stage setting box and user can select
any stage to delete or change its sequence.
User can select a certain stage and press its cycle number in the corresponding
Cycle
column, the cycle edit box will pop up as shown in figure E-3.3.
