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Gentier Real-Time PCR System
▶
▶
▶
II.
Temperature
: the temperature in °C, which is to be held for a defined time. User can use
and
keys, or manually enter the current step temperature in the
Temperature
input box.
Reminding
: the temperature setting range is 0.0°C -100.0°C.
Time
: the time for which the temperature is to be held. User can enter the current step
time in the
Time
input box.
Reminding
: the time setting range is 1s~60min.
Reminding
: If the current step is the last step of temperature program, user can check the
check box to set the current step time to infinite.
Ramp
: the rate of temperature change(°C) per second. User can use
and
keys, or
manually enter the temperature change rate in the
Ramp
input box.
Reminding
: the ramp setting range is 0.1°C/s~6.1°C/s.
Touchdown Step Mode
: the touchdown step mode allows the temperature program to
change the annealing step temperature from the initial temperature to the target
temperature as the cycling proceeds. User could set the corresponding parameters in the
Step Setting
dialog box, as shown in figure 5.6b.
Figure D-5.6b Step Setting Dialog Box – Touchdown Step Mode
▶
Initial Temperature
: the initial value of annealing temperature change range.
▶
Target Temperature
: the target value of annealing temperature change range.
▶
Delta Temperature
: the temperature change(°C) per cycle.
▶
Start Cycle
: the cycle number after which the temperature change is started.
