108
Gentier Real-Time PCR System
5)
Reminding
: the temperature center setting range is 35.5°C ~99.5°C.
Reminding
: the temperature offset setting range is 0.5°C ~20.0°C.
Reminding
: the other parameter settings are same with the standard step mode.
Reminding
: the Gentier 96S/96C instrument model cannot support gradient PCR step
mode.
If the current step is the last step of a melting stage, the step setting window of this step is
as shown in figure E-3.8.
Figure E 3.8 Step Edit window Melting step mode
▶
The melting stage allows the instrument system to read fluorescence signals after each
temperature increment. User can press the input boxes of
Increment
to edit the
temperature increment for the current melting step, as shown in figure E-3.8a.
Figure E-3.8a Step Edit window - Melting step parameter setting
Increment
: the temperature change(°C) after which the system will read the fluorescence.