Drawell Gentier 96C, Руководство пользователя

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Gentier Real-Time PCR System
curve diagram, or click the right mouse button to zoom out.
II. Melting Peak
: display the first negative derivative of the fluorescence intensity (-dF/dT)
respect to the corresponding temperature, as shown in the figure 13.2. If there is only a
single melting peak, it means that no non-specific PCR products are detected in the
experiment.
Figure D-13.2 Melting Curve analysis interface - Melting Peaks functional module
▶
Parameter introduction
:
X axis represents the temperature value;
Y axis represents the first negative derivative of the fluorescence intensity against the
corresponding temperature;
▶
Function key introduction
:
User can select the melting peak display mode from the drop-down list on the bottom left of
Melting Peaks
functional module. The melting curve can be displayed according to
Well
Color
,
Dye Color, Sample Color
and
Gene Color
.
The introduction of
< Y-axis Coordinate Adjustment >
and
< Export >
keys are
same with those keys introduced in
D-3.3.4.1 Abs Quant - Amplification Curve
.
< PeakArea >
: user can click
< PeakArea >
icon to define the melting peak area;
each side ofthe rectangle delimits the temperature and fluorescence range of the peak area.
Melting Curve Analysis Setting