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IX

Gentier Real-Time PCR System

Reminding

: the working environment of the Gentier instrument should be under normal

atmosphere (the altitude should below 2000m).

V. Biological Safety and Precautions

Biohazard

: the sample objects of the Gentier instrument are nucleic acids, please consider

all samples are with potential biohazard, and please take applicable safety protection
measures and wear appropriate protective goggles, clothing and gloves while processing
the samples.

Biohazard

: in case of any liquid overflows during the operation, please immediately

disinfect the contaminated area with appropriate detergent to avoid the spreading of
contaminant, prevent the laboratory or instrument from biohazards.

Biohazard

: please comply with the local or national applicable regulations to complete the

disposal of waste samples and contaminated materials.

Biohazard

: user should consider the abandoned Gentier instrument as biological

contaminated material and comply with the local or national applicable regulations to
complete the instrument disposal. Before disposal or recycle the instrument, please
completely clean and disinfect the instrument.

Biohazard

: user should only use reagents and consumables that are within their expiration

date.

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Содержание Gentier 96C

Страница 1: ...ted Chongqing Drawell Instrument CO Ltd Add Suite 2705 Building No 12 Shiyou Road No 1 Yuzhong District Chongqing China Tel 0086 023 63268643 Shanghai Drawell Scientific Instrument Co Ltd Add Suite 11...

Страница 2: ...I Gentier Real Time PCR System Gentier Real Time PCR System User Manual Drawell International Technology Limited...

Страница 3: ...le real time measuring nucleic acid signals from DNA binding fluorescent dyes or labeled probes and converts them to comparative quantitative readouts of DNA or reverse transcribed RNA Special Declara...

Страница 4: ...t is prohibited to stack more than 4 layers for the storage and transportation of packages Recycle indicates the packaging materials are recyclable Safty Labels on Gentier Instruemt Label Description...

Страница 5: ...t may results in instrument damage or abnormal function even constitutes a personal injury hazard Conventions Used in This Manual Convention Meaning Ordered list Procedure steps must be performed foll...

Страница 6: ...damage even personal injury Reminding please pay attention to the descriptions with Caution Reminding Prohibit symbols and the safety labels on the instrument and transport package I General Instrume...

Страница 7: ...f the instrumnet Move or lift the instrument in an improper way may result in bodily injury pain or instrument damage Prohibit never touch the plug the power cord or the power switch with wet hands Hi...

Страница 8: ...ces where people move around Caution the fuse tube type of the Gentier instrument is 250V F10AH located in the fuse tube box near the power outlet at the rear of the instrument Use improper fuse tube...

Страница 9: ...g the operation please immediately disinfect the contaminated area with appropriate detergent to avoid the spreading of contaminant prevent the laboratory or instrument from biohazards Biohazard pleas...

Страница 10: ...re Characters 6 3 3 Reagent Specifications 7 3 4 Consumable Specifications 7 B GENTIER INSTRUMENT INSTALLATION 8 1 UNPACKING INSTRUCTIONS 8 1 1 Unpacking Steps 8 2 WORKING ENVIRONMENTAL REQUIREMENTS 1...

Страница 11: ...Quant 61 3 3 4 2 Rel Quant 73 3 3 4 3 Melting Curve 77 3 3 4 4 High Resolution Melting 81 3 3 4 5 Genotyping 86 3 3 4 6 End Point Fluorescence 90 E INSTRUMENT SOFTWARE OPERATION 93 1 BOOT SCREEN MAIN...

Страница 12: ...ple Block 121 2 INSTRUMENT MAINTENANCE OPERATION INSTRUCTIONS 122 2 1 Maintain SufficientAir Flow 122 2 2 Maintain Stable Power Supply 122 2 3 Maintain Instrument Cleanliness 122 2 4 Replace Fuse Tube...

Страница 13: ...in fields of immunology molecular biology forensic science genetics archeology zoology phytology etc 2 Product Constitute and Instrument Structure 2 1 Product Constitute The Gentier real time PCR syst...

Страница 14: ...x 11 Internet access 3 Loading platform 7 Power switch 10 12 USB interface 13 Removable air vent cover 3 Product Introduction 3 1 Instrument Parameters and Characters 3 1 1 Instrument General Paramete...

Страница 15: ...tocol Ethernet connection Gentier Real Time PCR System USB interface Reminding Connected to IEC EN 60950 1 approved computer host and mouse Application Environment Temperature 10 C 30 C Relative humid...

Страница 16: ...ent Models 96E 96S 96R 96C 1 FAM SYBR Green I etc 2 VIC HEX TET JOE etc 3 ROX Texas Red etc 4 Cy5 etc 5 Alexa Fluor 680 etc 6 FRET Probe Reminding channel 6 is for FRET probe which takes the report gr...

Страница 17: ...the Gentier instrument Main Control Computer the recommend configurations of main control computer are listed as below Computer Processors quad core CPU Internal Storage 8GB Hard Disk 500GB Network Ca...

Страница 18: ...tantaneous power off protection function which can protect all configuration settings in case of power off and the interrupted experiment can continue after power on Multiple PCR Step Modes Gentier in...

Страница 19: ...n 99 steps and the maximum cycle number is 99 User can set the program for low temperature long term preservation after the experiment Report Template Design the unique universal report function is de...

Страница 20: ...well supported by protective foams Figure B 1 Package Details 1 1 Unpacking Steps The unpacking of the Gentier transport package mainly includes 3 steps as shown in figure B 2 The specific operation...

Страница 21: ...ent on both sides take it out of the carton and place it on steady plane shown in figure B 2e B 2f Figure B 2 Unpacking Steps Reminding in order to prevent the formation of condensation water please d...

Страница 22: ...Please also keep instrument related documents provided by our company for future use too 2 Working Environmental Requirements The Gentier instrument is for indoor use only 2 1 Instrument Space Require...

Страница 23: ...rument General Parameters use improper power may damage the circuit system and cause fire it is recommended to use UPS power supply Reminding before connecting the AC power supply please ensure the co...

Страница 24: ...e removable air vent cover as shown in figure B 3b 2nd Step the transport lock screw is fixed upon two cooling fans as shown in figure B 3c Please use screwdriver to remove the fixed transport lock sc...

Страница 25: ...13 Gentier Real Time PCR System Figure B 3 Remove transport lock...

Страница 26: ...terface will automatically pop up please follow the prompt to install the software 4th step double click the MED TL icon on the desktop to startup the Gentier aplication software 3 4 Computer Network...

Страница 27: ...he control computer subnet mask should be identical to the Gentier instrument subnet mask Default gateway the control computer deault gateway should be identical to the Gentier instrument deault gatew...

Страница 28: ...rument will enter the standby state Caution before power on the Gentier instrument please ensure The transport lock of the Gentier instrument is removed The external devices of the Gentier instrument...

Страница 29: ...ep place the cosumables that contain the sample and PCR reagent mixture on the sample block and press the Open Close function key again to withdraw the loading platform 3rd Step select or edit an expe...

Страница 30: ...instrument and software interface figures presented herein are all based on Gentier 96E instrument model For different Gentier instrument models the software interface will display the corresponding...

Страница 31: ...quick start bar on this interface as shown in figure D 2 Figure D 2 Startup interface of application software 2 Quick Start Bar The quick start bar of application software is as shown in figure D 2 1...

Страница 32: ...me in the drop down menu and then click Login to log in as the current user account as shown in figure D 2 1a Reminding the application software provides two user names by default user and admin Figur...

Страница 33: ...ent with the logged in user account name and experiment creation time Reminding the experiment name can consist of numbers letters horizontal lines underlines or Chinese characters but cannot contain...

Страница 34: ...the application software again after setting the default instrument the application software will automatically connect the default instrument and display its status User can check the Display at Sta...

Страница 35: ...riment N create a new experiment 2 New Experiment from Existed Experiment user can select a saved experiment file and create a new experiment file with same experiment settings as the selected experim...

Страница 36: ...nd manage the experiment report information after the experiment 12 Exit exit and close the application software View V submenu includes three options Options and Functional Description 1 Quick Start...

Страница 37: ...instrument which is Ready or Running Tool T submenu include three options Options and Functional Description 1 Instrument Management Click Instrument Management option in Tool submenu and the applica...

Страница 38: ...ent List User can double click a certain instruemnt IP address in the Instrument List and the selected IP address will be displayed in the IP Address input box on the right click Test and the applicat...

Страница 39: ...ent Information Area display the relevant information of instrument selected in the Instrument List such as Name IP Address Model Serial No and Version No of instrument components etc Computer informa...

Страница 40: ...ocal Directory the Local File list will display all experiment files and data files in this directory c Instrument File display all experiment files and data files saved in the connected instrument Cl...

Страница 41: ...input the salt concentration value in Salt Concentration input box by using the keyboard or keys User can click Calculate and the application software will automatically calculate Fwd Primer Tm value...

Страница 42: ...ar Update Report user can click Update Report to refresh the information in the research report Save Research Report after editing the research report please click Save icon to choose a path for savin...

Страница 43: ...re D 3 5a Option submenu Configuration Management interface Default Path tab Interface Introduction and Functional Description Default Path tab the configuration management interface dispalys the Defa...

Страница 44: ...efined standard temperature templates are displayed in this tab as shown in figure D 3 5b These templates are provided by the application software for temperature program stage settings Figure D 3 6 C...

Страница 45: ...ly click test to check whether the PANA instrument can be connected as shown in figure D 3 5c Figure D 3 5c Configuration Management interface PANA tab 2 User click User option in the Option submenu a...

Страница 46: ...ave to save the current general user permission settings b If user login as general user account user can view the general user permissions in this tab 3 Choose Language click Choose Language option i...

Страница 47: ...a new experiment file with same experiment setting as the selected experiment file Open Data File open a data file for viewing or analyzing Save Experiment the experiment file is saved to the default...

Страница 48: ...the experiment file tab at the bottom of operation area and user can select or close any experiment file in this tab Reminding if several experiment files are created or opened at the same time user...

Страница 49: ...pop up choose sample parameter template window user can choose a saved sample parameter template and set current experiment based on this template Save Sample Parameter Template click this icon in too...

Страница 50: ...Figure D 5 Run Setting interface Run Setting The Run Setting interface consists five parts temperature program area stage and step lists experiment and step editing areas 1 Temperature program area us...

Страница 51: ...is show below the solid blue line The solid blue line between two stages steps represents the temperature ramp or increment or readings of melting step If user decide to read the fluorescence at a cer...

Страница 52: ...Lid user can enter the hot lid temperature for current experiment in the Hot Lid input box User can also check the Open check box to determine whether to use the hot lid heating function Reminding the...

Страница 53: ...e User can click Delete icon to delete the selected stage User can click Up icon to move up the selected stage User can click Down icon to move down the selected stage Reminding User can also click ic...

Страница 54: ...e please click Close to return to the run setting interface The new stage will be added to the temperature program area and stage list Reminding at least one stage should be included in the temperatur...

Страница 55: ...ted step as shown in figure D 5 6a Figure D 5 6a Step Setting dialog box Standard step mode Introduction to Step Setting Dialog box Step Mode user can set the step mode for the selected step in the dr...

Страница 56: ...hange C per second User can use and keys or manually enter the temperature change rate in the Ramp input box Reminding the ramp setting range is 0 1 C s 6 1 C s Touchdown Step Mode the touchdown step...

Страница 57: ...g Dialog Box Long Step Mode IV Initial Time the initial value of the elongation time change range Target Time the target value ofthe elongation time change range Delta Time the time change per cycle S...

Страница 58: ...er 96S and Gentier 96C instrument models Reminding the temperature center setting range is 35 5 C 99 5 C Reminding the temperature offset setting range is 0 5 C 20 0 C Details User can click Details t...

Страница 59: ...increment in the Increment input box Reminding the temperature increment setting range is 0 1 C 5 0 C VI Continuous Melting continuous melting stage allows the system to read the fluorescence more fre...

Страница 60: ...area as shown in figure D 6 Figure D 6 Sample Setting interface Sample Setting Operation 1 Sample Setting Area After entering the sample setting interface user shall first choose a sample well in the...

Страница 61: ...nd click corresponding column number to select multiple discontinuous columns of wells Press Ctrl key on computer keyboard and click corresponding row number to select multiple discontinuous rows ofwe...

Страница 62: ...to select a data analysis color for all samples with the current sample name Sample Type user can set the relevant sample type for the selected wells from the Sample Type drop down list The optional s...

Страница 63: ...rd Conc user can select a single standard sample and enter its standard concentration in Standard Conc input box Conc Unit user can select a single standard sample and set its concentration unit from...

Страница 64: ...ries of diluted standard samples from Dye drop down list User can select the number of standard samples from Replicate drop down list User can select the auto concentration setting direction from Dire...

Страница 65: ...icate group number setting c The application software can automatically divide multiple replicate groups according to user requirements Please first select all wells of same samples in the sample sett...

Страница 66: ...in the sample setting area and set the unique ID for the selected well in the Unique ID input box click Apply to confirm the unique ID User can also select multiple wells in the sample setting area a...

Страница 67: ...ck Default to clear all QC failure condition settings Clear Selected Wells user can click icon to clear the relevant property settings for all selected wells 3 3 3 Run Monitoring After finishing the e...

Страница 68: ...Setting Run Info by default Reminding user can select the functional module displayed on the run monitoring interface from the drop down list on the top right corner of each area Reminding user can cl...

Страница 69: ...gram Please click Y axis Coordinate Adjustment icon and the coordinate range setting box will pop up automatically as shown in figure D 7 1a User can select Automatic or Manual option in the coordinat...

Страница 70: ...ring interface Temperature Program function module Parameter introduction same with the parameters introduced in D 3 3 1 Run Setting Temperature program area Function key introduction user can click E...

Страница 71: ...ce and Sample Info function modules 4 Run Info display the current instrument information and the real time running status such as the Stage Step Cycle and Remaining Time of of the current experiment...

Страница 72: ...nterface Heat Map function module 3 3 4 Analysis After experiment running user can click the Analysis tab to enter the analysis interface According to the experiment settings and requirements user sha...

Страница 73: ...f an interest nucleic acid Samples with unknown initial nucleic acid quantities are amplified as well as a dilution series of gene specific standard samples with known concentrations The measured Ct v...

Страница 74: ...op down list on the top right corner of each area Reminding user can click the Full Screen icon on the top right corner of each area to display the current functional module in full screen Figure D 9...

Страница 75: ...ne Color Y axis Coordinate Adjustment user can adjust the Y axis coordinate of the amplification curve diagram Please click Y axis Coordinate Adjustment icon and the coordinate range setting box will...

Страница 76: ...er the mouse over a certain amplification curve the curve will be highlighted and its corresponding channel and well coordinate will be displayed on the right of cursor II Standard Curve displays the...

Страница 77: ...2 display the linear regression coefficient square value of standard curve Y axis intercept display the Y axis intercept of standard curve Function key introduction Load Std Curve click Load Std Curv...

Страница 78: ...ear the standard curve displayed on the Standard Curve functional module Save Std Curve user can click Save Std Curve and enter a name in the enter name dialog box for the standard curve file to save...

Страница 79: ...Result sub tab is selected by default on the result table functional module which display the sample details and results of the current experiment as shown in figure D 9 4a Figure D 9 4a Result Table...

Страница 80: ...V Raw Curve display the diagram of raw amplification curve without subtract baseline as shown in figure D 9 5 The parameters and function keys of raw curve functional module are same with those of amp...

Страница 81: ...e wells Function key introduction User can slide the Cycle slider at the bottom of raw fluorescence functional module to view the raw fluorescence value of different wells at different cycles Y axis C...

Страница 82: ...dition set on the Sample Setting interface then the relevant sample well will be displayed as N A on QC diagram Abs Quant Analysis Setting User can click the Analysis Setting icon in the Tool Bar and...

Страница 83: ...rt Cycle and End Cycle for All Selected Rows on top of the Baseline list User can click Restore on the top right corner of Baseline list to restore the Start Cycle and End Cycle ofthe automatic baseli...

Страница 84: ...ing dye a User can check a certain check box in the Remove column and click OK to remove the experiment data ofthe selected target gene and its marking dye b Click Cancel to cancel opertaion and retur...

Страница 85: ...tification analysis interface consists of eight functional modules Amplification Curve Bar Chart Sample Setting Result Table Standard Curve Raw Curve Raw Fluorescence and Heat Map This interface is di...

Страница 86: ...sample for comparison Y axis shows the Relative Quantity ratio of the compared gene and sample Reminding the application software set the relative quantity ratio of the reference sample to 1 by defau...

Страница 87: ...pression option the Bar Chart functional module will display the relative quantity ratio of target gene sample and reference gene sample d Sort User can use and keys to set the display sort order of B...

Страница 88: ...ing dye labeled genes from the Analysis interface c User can click a certain Eficiency column use and keys to set the amplification efficiency ofthe corresponding dye labeled gene Reminding if a stand...

Страница 89: ...ith the rising temperature Different dsDNA has different melting temperature Tm the temperature at which 50 of the DNA has dissociated from double stramded to single stranded owing to different base s...

Страница 90: ...alue Y axis represents the Relative Fluorescence Unit RFU Function key introduction User can select the melting curve display mode from the drop down list on the bottom left of Melting Curve functiona...

Страница 91: ...ue Y axis represents the first negative derivative of the fluorescence intensity against the corresponding temperature Function key introduction User can select the melting peak display mode from the...

Страница 92: ...igure D 13 3 Figure D 13 3 Melting Curve Analysis Setting interface Melt Curve tab Dye display the settings of Dye and its marked Gene of the current experiment as shown in figure D 13 3 PeakArea disp...

Страница 93: ...t the difference of melting temperature Therefore high resolution melting curve is often used for single nucleotide polymorphism SNP analysis mutation scanning methylation research and genotyping etc...

Страница 94: ...tical sliders on the left are the Pre melting Range threshold lines which are used to specify the pre melting temperature range while two vertical sliders on the right are the Post melting Range thres...

Страница 95: ...ils please refer to D 3 3 4 4 High Resolution Melting High Resolution Melting Analysis Setting Figure D 14 2 HRM analysis interface Normalized Melting Curve functional module III Diference Plot displa...

Страница 96: ...unctional module High Resolution Melting Analysis Setting User can click the Analysis Setting icon in Tool Bar and the application software will automatically pop up the high resolution melting analys...

Страница 97: ...ge user can use and keys to set the pre melting temperature range of the normalization area of the melting curve b Post Melt Range user can use and keys to set the post melting temperature range of th...

Страница 98: ...roup gene for the Diference Plot functional module II Gene and Sample tab the introduction of relevant parameters and function keys is same with those introduced in D 3 3 4 1Abs Quant Abs QuantAnalysi...

Страница 99: ...typing analysis interface Interface Introduction and Parameter Description I Scatter Plot display the end point fluorescence distribution of two dyes as shown in figure 16 1 Figure D 16 1 Genotyping a...

Страница 100: ...y the sample details of and result data of the current experiment as shown in figure 16 2 Figure D 16 2 Genotyping analysis interface Result Table functional module User can double click the title of...

Страница 101: ...nt analytical method in the genotyping analysis setting window I Genotyping tab user can set Analysis Mode Reference Dye Baseline and Gene Group Parameter in Genotyping tab as shown in figure D 17 Fig...

Страница 102: ...Quant Abs QuantAnalysis Setting 3 3 4 6 End Point Fluorescence The end point fluorescence analysis displays the final detection result based on the fluorescence intensities measured at the end point...

Страница 103: ...intensities measured at end the point of amplification plateau phase as shown in figure 18 1 Figure D 18 1 End Point Fluorescence analysis interface End Point Fluorescence Scatter Plot Parameter intro...

Страница 104: ...n the end point fluorescence analysis setting window I End Point Fluorescence tab as shown in figure D 19 Figure D 19 End Point Fluorescence Analysis Setting interface End Point Fluorescence tab Analy...

Страница 105: ...the power switch of Gentier instrument The instrument system will automatically conduct self inspection the touch sceen will light up and display instrument software boot screen After self inspection...

Страница 106: ...rument hardware or software error has occured cannot execute any operation System Time display the current system date and time 2 Operation Area includes Experiment File Run Setting Run Monitoring Gen...

Страница 107: ...tem software includes Experiment File Run Setting Run Monitoring General Setting four tabs User could perform relevant operations and set the corresponding contents according to the specific experimen...

Страница 108: ...nt File interface Interface Introduction Parameter Descriptions 1 Experiment File Displaying Area display the pre existed experiment files or file folders within the instrument system as shown in figu...

Страница 109: ...tab Experiment file information window 3 Experimental file action bar consists of New Rename Delete and USB four keys as shown in figure E 2 3 Figure E 2 3 Experiment File tab Experimental file actio...

Страница 110: ...options in the drop down list Export Experiment export the selected experiment file or file folder from the instrument system to the USB device Import Experiment import the selected experiment file or...

Страница 111: ...ab is selected on the run setting interface by default as shown in figure E 3 User can edit the relevant experiment settings and temperature program in the Temperature Setting sub tab Interface Introd...

Страница 112: ...Temperature display the target temperature ofthe current step Time display the temperature holding time of the current step Fluor display whether to read the fluorescence at the current temperature s...

Страница 113: ...ation Melting Continuous Melting and Cooling User can also press Custom Stage to define the stage setting according to specific experiment requirements User can press Add to add the selected stage to...

Страница 114: ...step to delete or change its order Reminding at least one step should be included in one stage Step 4 Set fluorescence reading step In the step setting box user can press the Fluor coloum of a cetain...

Страница 115: ...ep Edit window Standard step mode 1 The Standard step mode option is selected by default User can press the input boxes of Temperature Time and Ramp to edit the corresponding parameters for the curren...

Страница 116: ...Figure E 3 5 Temperature Setting sub tab Step Edit window Touchdown step mode The touchdown step mode allows the temperature program to change the annealing step temperature from the initial temperatu...

Страница 117: ...temperature change is started Reminding the initial and target temperature setting range is 35 0 C 100 0 C Reminding the delta temperature setting range is 0 1 C 5 0 C Reminding the star cycle range i...

Страница 118: ...l value of the elongation time change range Target Time the target value of the elongation time change range Delta Time the temperature change C per cycle Start Cycle the cycle number after which the...

Страница 119: ...he system will automatically calculate the gradient temperatures User can press the input boxes of Temp Center and Temp Offset to edit the corresponding parameters for the current gradient step User c...

Страница 120: ...ep is the last step of a melting stage the step setting window of this step is as shown in figure E 3 8 Figure E 3 8 Step Edit window Melting step mode The melting stage allows the instrument system t...

Страница 121: ...ntinuous Melting step mode Continuous melting stage allows the instrument system to read fluorescence more frequently user can press the input boxes of Readings to set the fluorescence reading times p...

Страница 122: ...rent experiment in the Fluorescence Setting sub tab User can check the corresponding check box in the Channel column to set the fluorescence channel for the current experiment 1 6 channels for Gentier...

Страница 123: ...n Monitoring interface 1 Run Status Bar displays the real time running status of the the current experiment includes Remaining Time Stage Cycle and Step 2 Run Monitoring Option Bar user can select Flu...

Страница 124: ...Diagram Real time Amplification Curve X axis represents the cycle number Y axis represents the Relative Fluorescence Unit RFU User can press any dye under the Fluorescence option in the run monitoring...

Страница 125: ...3 Run Monitoring Diagram Heat Map The fluorescence heat map displays the 96 sample wells corresponding to the sample block The color bar on the right side of fluorescence heat map displays the change...

Страница 126: ...General Setting The General Setting tab consists of Instrument Configuration and Service three sub tabs 2 4 1 Instrument Sub tab The General Setting tab displays the Instrument sub tab by default as...

Страница 127: ...ction display the latest self inspection result of the current Gentier instrument user can press Details to view detailed self inspection information Alarm Information user can press Details to view t...

Страница 128: ...b tab Introduction to Configuration Sub tab Network Information display the network information of the current Gentier instrument User can press Edit and the instrument software will pop up the networ...

Страница 129: ...the touch screen sound Current Date Time user can press Set to set the date and time displayed on the current Gentier instrument the date time setting window will pop up as shown in E 6 2b Figure E 6...

Страница 130: ...rovided are English and Simplified Chinese 2 4 3 Service Sub tab Figure E 6 3 General Setting tab Configuration sub tab Introduction to Service sub tab Lock Instrument for Transportation user can pres...

Страница 131: ...our professional engineer to get the relative information in case the instrument require maintenance Build No user could press Details to view the build number of the current instrument Debug Mode pr...

Страница 132: ...ck when the instrument is electrified Caution ethanol is a flammable and volatile liquid it s exposure may irritate eyes skin and respiratory tract and may lead to central nervous system hypofunction...

Страница 133: ...do not spray the detergent directly on the touch screen as malfunctions ofthe electronics or may occur Caution do not use abrasive detergent or rough material as they may scratch the touch screen 1 3...

Страница 134: ...nt 2 3 Maintain Instrument Cleanliness Contamination of loading platform sample block or optical parts can interfere with thermal cycling and data collection Avoid contaminating the Gentier instrument...

Страница 135: ...e F 1 Replace the fuse tube Reminding in case there is no display on the screen after starting up the Gentier instrument please check the fuse tube Caution improper fuse tube may lead to circuit syste...

Страница 136: ...ing and medical disposable gloves Open the loading platform and get all consumables out of the sample block Switch offthe instrument and unplug the power cord Prepare the detergent lab routine disinfe...

Страница 137: ...l as shown in figure F 3a 2nd Step the transport lock screw should be fixed upon two cooling fans as shown in figure F 3b Please use screwdriver to fix the transport lock screw as shown in figure F 3c...

Страница 138: ...transportation of Gentier instrument can adopt general transport with awnings The specific packing steps of Gentier instrument are as follows 1st Step pack the Gentier instrument with plastic bag as s...

Страница 139: ...127 Gentier Real Time PCR System Figure F 4 The packing steps of Gentier instrument...

Страница 140: ...ate power voltage Adjust the scope of power voltage into a normal range Fuse tube damaged Replace the fuse tube Others Contact us 2 Boot screen display error message The activation of system failed Pl...

Страница 141: ...ly contact with our company Caution in case any of following situation occurs please immediately cut off the power supply and contract us We will arrange qualified maintenance personnel for processing...

Страница 142: ...ngqing Drawell Instrument CO Ltd Add Suite 2705 Building No 12 Shiyou Road No 1 Yuzhong District Chongqing China Tel 0086 023 63268643 Shanghai Drawell Scientific Instrument Co Ltd Add Suite 1117 Lane...

Страница 143: ...O Ltd Add Suite 2705 Building No 12 Shiyou Road No 1 Yuzhong District Chongqing China Tel 0086 023 63268643 Shanghai Drawell Scientific Instrument Co Ltd Add Suite 1117 Lane561 XiuChuan Rd PuDong New...

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