Kendro HERAcell 150, Operating Instructions Manual

Page: 23 / 251

CO
2
Incubator HERAcell ® 150 Operating Instructions
23
4. Description of the device
For humidification, processed water of the following quality is required:
• distilled,
or
• demineralized and destilled/autoclaved,
or
• deionized and destilled/autoclaved.
CO
2
supply:
To ensure the growth conditions for the cell and tissue cultures, the work spa-
ce is supplied with CO
2
.
The pH of the bicarbonate-buffered culture media largely depends on the CO
2
content of the work space atmosphere.
The CO
2
content of the work space atmosphere can be controlled within a range
of 0-20 %.
The supplied CO
2
must have one of the following quality characteristics:
• Purity 99,5 % min.,
• medical gas quality.
O
2
supply:
If the O
2
incubator is to operated with more than 21 % oxygen, the work spa-
ce is supplied with oxygen. The O
2
content of the work space atmosphere can
be controlled within a range of 21-90 %.
N
2
supply:
If the oxygen content during operation is to be lowered to less than 21 % (air
oxygen content), the work space is supplied with nitrogen. The O
2
content of
the work space atmosphere can be controlled depending on the configuration
of the sensor in a range of 1 % to 21 % or of 5 % to 21 %.
4.5 ContraCon decontamination routine
The ContraCon decontamination routine is used to decontaminate the complete
work space including all installed components and sensors.
During this routine, a moist and wet atmosphere with highly decontaminating
effect is created for 9 hours at a temperature of 90° C.
The effectiveness of the ContraCon decontamination routine has been tested
and certified by independent institutes. Information about these tests is available
at request from Kendro Laboratory Products.
The entire program run of the ContraCon decontamination routine will take approx
25 hours.
After the run has been completed, the device must be reactivated using the auto-
start routine.
NOTE – Thermal protection
If the thermal protection for the device re-
sponds, the ContraCon decontamination rou-
tine can only be be started after the cause of
the failure has been repaired or reset (see
Section 6.13).