Kendro HERAcell 150 Operating Instructions Manual Download Page 1

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Sicherheitswerkbank HERAsafe HS 9/12/15/18

Valid: 07.2004 / 50075549 B

incubator HERAcell

150

with decontamination routine

Operating Instructions

Summary of Contents for HERAcell 150

Page 1: ...Sicherheitswerkbank HERAsafe HS 9 12 15 18 Valid 07 2004 50075549 B CO2 incubator HERAcell 150 with decontamination routine Operating Instructions...

Page 2: ...not apply to reproductions for in plant use The contents of the operating instructions are subject to change without further notice For translations into foreign languages the German version of these...

Page 3: ...17 3 3 Space requirements 18 3 4 Transport 18 3 5 Stacking 19 3 6 Retrofitting Modifications 19 3 7 Tray 19 4 Description of the device 20 4 1 Front view 20 4 2 Rear view 21 4 3 Safety devices 22 4 4...

Page 4: ...ction 58 9 1 Decontamimation procedures 58 9 2 Wipe Spray disinfection 58 9 3 ContraCon decontamination routine 62 9 4 Activating the ContraCon decontamination routine 65 9 5 Cancelling the ContraCon...

Page 5: ...tion installation 30 Fig 16 Gas pressure hose installation 32 Fig 17 CO2 connection combined CO2 O2 N2 connection 32 Fig 18 Switchbox with gas guard 33 Fig 19 CO2 connection with gas guard 33 Fig 20 C...

Page 6: ...x Vertrieb Service 0800 1 112114 E Mail info kendro de Enquiries from Europe Middle East and Africa Tel 49 0 6184 90 6940 Fax 49 0 6184 90 7474 E Mail info kendro com Postal address USA Kendro Laborat...

Page 7: ...measures are to be taken in case of an accident Any repairs to the device must only be performed by adequately trained and authorized expert personnel The contents of the operating instructions are s...

Page 8: ...ing instructions WARNING is used if non observance may cause serious or even lethal injuries CAUTION is used if non observance may cause medium to minor injuries or damage NOTE is used for application...

Page 9: ...ce off Open device doors Leave device doors open for at least 30 seconds Check humidification system water level refill as necessary level between 1 2 l min and 3 0 l max Refill 300 ml water for Contr...

Page 10: ...ral notes Clean device work space Close device doors Remove samples and water from device Charge device Note in operating instructions page 1 3 3 Symbols on the device CE symbol VDE safety tested Test...

Page 11: ...56 Laboratories in the central area of clinics and hospitals The gases required for the incubator CO2 and or N2 O2 are supplied to the device from a separate gas supply system either from gas cylinder...

Page 12: ...otice of the trade association of the German chemical industry For other countries the applicable national regulations are binding 1 6 Safety notes on gases Carbon dioxide CO2 As CO2 is rated as a har...

Page 13: ...g materials WARNING Oxygen explosion O2 may explode in combination with oils grea ses and lubricants If highly compressed oxy gen comes in contact with grease or oilcon taining substances the mixture...

Page 14: ...the room atmosphere may cause suffocation due to oxygen deficiency If N2 is released initiate safety measures im mediately Leave the room immediately and do not allow others to enter the room Inform...

Page 15: ...on CO2 or CO2 O2 Incubator with 3 door gas tight screen and with continuous shelves optional Shelf 3 3 Support rail for shelf 4 4 Shelf supports for shelf 6 6 Tray 1 1 Insert for pressure compensation...

Page 16: ...livery 2 3 Acceptance inspection After the device has been delivered check the delivery immediately for completeness possible damages If damages are detected or if components are missing please contac...

Page 17: ...sive heat are not allowed near the location of the HERAcell 150 3 2 Room ventilation When CO2 O2 N2 is supplied the work space of the incubator is slightly pres surized The pressure is released throug...

Page 18: ...lled near the floor The height of the floor stand should not fall below 200 mm Several floor stands and carriers are available as options from Kendro part numbers see Section 11 1 Spare parts and acce...

Page 19: ...ations The outer door and the glass door can be equipped with left or right side fasteners The door fastening can also be reversed later The standard version can also be retrofitted with a three ele m...

Page 20: ...sor optional 7 Pressure compensation opening with insert 8 Measuring opening 9 Outer door 10 Outer door seal replaceable 11 Foot height adjustable 12 Gas humidification optional 13 Water level sensor...

Page 21: ...ation opening 2 Access port 42 mm 3 Stacking elements 4 Switchbox with supply interfaces for combined gas connection CO2 and O2 N2 with gas guard 5 Switchbox with supply interfaces for combined gas co...

Page 22: ...st be at least 3 C higher than this temperature The heating system controls the incubation temperature from this temperature threshold up to 55 C The principle of air jacket heating and the additional...

Page 23: ...red to less than 21 air oxygen content the work space is supplied with nitrogen The O2 content of the work space atmosphere can be controlled depending on the configuration of the sensor in a range of...

Page 24: ...en preprogrammed at the fac tory and cannot be changed It protects the stored cultures from overheating If the temperature is exceeded by more than 1 C the thermal protection responds and the work spa...

Page 25: ...hrough a sterile filter with a separation rate of 99 97 for a particle size of 0 3 m HEPA filter quality Gas guard The devices can be equipped with an optional gas guard The gas guard allows the conne...

Page 26: ...sur face thereby supporting both the prevention of contaminati on and the easy effective decontamination Interior container All components of the work space are made of stainless steel and have a bur...

Page 27: ...sings 3 in the water tray are used as indicators for the maximal level Heating system An air jacket heating is used for heating the work space The arrangement of the heating elements ensures that cond...

Page 28: ...in the operating room NOTE Operating conditions When accessories are to be operated in the work space of the CO2 incubator the ambient condition requirements must be observed see table below The ener...

Page 29: ...of the shelf system The support rails are secured using spring pressure After the support hooks have been inserted into the rail the shelves are pushed onto the support hooks Support rail installatio...

Page 30: ...shelf supports 3 Make sure that the shelf supports is positioned in the two tilt protections in a way that it can move freely Levelling the device 1 Position a bubble level onto the center shelf 2 Ro...

Page 31: ...ly to a range between 0 8 bar min and 1 0 bar max and make sure that this pressure setting cannot be changed NOTE Pressure compensation opening To ensure permanent pressure compensation the pressure c...

Page 32: ...sterile filter 4 Secure gas pressure hose to the sleeve of the sterile filter using the hose clamp 5 Make sure that the access port is sealed when it is not used 5 3 2 Installing devices without gas g...

Page 33: ...y to device work space CO2 connection with gas guard Fig 19 For a device that is operated with process gas CO2 and equipped with the optional gas guard connect the gas supply lines as follows The dist...

Page 34: ...upper connection 2 of the gas guard the secondary gas supply line 8 is connected to the lower connection 3 of the gas guard Upon delivery of the device a short gas pressure hose 5 connects the output...

Page 35: ...breaker G 16 Connection to the power supply system 1 Before connecting the device to the power supply check to see if the voltage of the power supply corresponds with the specifications on the namepl...

Page 36: ...llows 9600 baud 8 data bits 1 stop bit no parity Command sequences Data communication is achieved with a defined structure of command sequences frames Frame structure STX command data BCC ETX Command...

Page 37: ...e shows that there is no current failure Faulty response from control unit If a returned response is incomplete or faulty the CPU responds with an NAK 15H only 1 byte without frame Otherwise the comma...

Page 38: ...an alarm mes sage is issued to the connected alarm monitoring system The potential free con tacts 1 changeover contact have been laid out for the following circuits HERAcell alarm relay NOTE Switching...

Page 39: ...he connecting cable is comprised in the scope of delivery The values for the operating voltage of the external circuits and of the fusing of the alarm system are given in the table below 1 Connect the...

Page 40: ...key for setting the O2 no minal value and the O2 display are not installed on the ope rating panel Fig 24 Power switch Fig 25 Operating panel functions 1 Temperature display 2 Heating LED 3 Key for se...

Page 41: ...umber for the corre sponding assembly parameter set P 1 Operating and display board P 2 Measuring cell P 3 Main board P n Parameter number The CO2 display shows the software version device version 2 T...

Page 42: ...perature display shows the current nominal value 2 Enter the nominal value The nominal value can be increased or reduced in increments if you keep the key depressed the UP DOWN function switches to a...

Page 43: ...in increments if you keep the key depressed the UP DOWN function switches to a rapid increase reduction after approx 3 seconds another increase reduction occurs To increase the nominal value Press th...

Page 44: ...ppears The oxygen supply system is now disabled 6 7 Enabling the O2 control The O2 control can be reenabled at any time during the operation 1 Enable the control function Press the keys or the keys Se...

Page 45: ...the nominal value Release both keys The CO2 display shows the current nominal value measured in the work space 6 9 Setting the high low humidity If condensate forms at the culture containers due to h...

Page 46: ...The temperature display shows the current mode high humidity 3 Change the mode Use the following key combinations to switch between the two modes Press the keys or Press the keys The temperature displ...

Page 47: ...peration of the device The auto start routine should be run at least every three months on the occa sion of cleaning and maintenance works Running the routine usually takes 5 to 7 hours At low room te...

Page 48: ...ff 5 Cancel the auto start routine Keep the depressed for 5 seconds The displays returns to normal operation incubation operation NOTE Cancelling the routine The auto start routine can be cancelled an...

Page 49: ...f the device available when contacting Technical Service NOTE Response delay To prevent short time changes of the operating conditions from resulting in repeated failure messages during the operation...

Page 50: ...the system detects a failure the display that is assigned to the corre sponding control circuit shows a failure code Example If the tempera ture display shows failure code 101 a failure in the tempera...

Page 51: ...e temperature display shows the 10 failure codes that had been regi stered last The CO2 display shows the ranking of the individual failure codes within the table 6 Exit the failure code table Release...

Page 52: ...e 88 Failure upon auto start Total time elapsed or max adjustment value exceeded Repeat auto start 99 Device doors open Doors open for more than 10 minutes door switch Close device doors test door swi...

Page 53: ...and Pressure drop in both monitored gas cylinders A and B pressure 0 6 bar and Code Description Cause Repair 300 O2 below nominal value Act val nom val 1 No O2 Prepressure low Supply line blocked Che...

Page 54: ...he power switch NOTE Thermal protection When the cause of the failure e g excessive temperature in the operating room has been repaired the device is set to normal incubation operation after it has be...

Page 55: ...s must be installed safely The optional gas humidification must be connected to the device integral gas supply and aligned parallel to the rear panel NOTE Hygiene regulations Prior to any operation th...

Page 56: ...perature control adjusts the temperature to the set nominal value humidity rises 9 When temperature and relative humidity are constant the automatic adju stment of the CO2 O2 measuring system is perfo...

Page 57: ...ml of fresh processed water and start the ContraCon decontami nation routine After the ContraCon decontamination routine has been run disconnect the device from the power and gas supply 4 Wipe device...

Page 58: ...anual disinfection pro cedure for the device and for all accessories 9 2 Wipe Spray disinfection The wipe spray disinfection is carried out in three stages Predisinfection cleaning final disinfection...

Page 59: ...taining disinfectants ZH 1 598 Kendro Laboratory Products recommend the disinfectant Barrycidal 36 When applied properly Barrycidal 36 is a highly effective broad range disinfectant The effectiveness...

Page 60: ...aning and disinfecting always obser ve the safety instructions and hygiene regulations Wear safety gloves Wear safety goggles Wear mouth and respiratory system protec tion gear to protect the mucous m...

Page 61: ...2 2 If required remove the blower wheel and its cover from the baseplate of the measuring cell The wheel and the cover can be autoclaved Removing blower wheel and cover 1 Fig 26 Remove the two retaini...

Page 62: ...er completion of the run always wear safety gloves when touching these surfaces observe the warning indicator at the operating panel The entire program run of the decontamination routine takes approx...

Page 63: ...ure nominal value 2 C Heating phase Remaining run time approx 25 hours The work space is heated to a temperature of 90 C while an elevated relative humidity is created The current decontamination temp...

Page 64: ...has elapsed to 0 hours the device has reached the originally set working temperature again e g 37 C The ContraCon de contamination routine must then be ended by pressing the appropriate key NOTE Overt...

Page 65: ...acoustic alarm sounds after 30 seconds 3 Start the ContraCon routine Keep the key depressed for 5 seconds The ContraCon routine LED flashes 4 Close the device doors The actual value is shown at the t...

Page 66: ...Electrical safety check in accordance with the relevant national regulations e g VBG 4 NOTE Functional check If safety devices were removed or disabled for inspections the device must not be operated...

Page 67: ...he comparison measurement NOTE Isothermal container Do not use a container filled with water as an isothermal container as the evaporation of water will result in a lower temperature reading Compariso...

Page 68: ...the key The preset value of 37 C is displayed 3 Enter the measured value destination value Press the keys or Press the keys Destination value e g 36 4 C 4 Accept the destination value Press the key T...

Page 69: ...ng sample is withdrawn through the sealable mea surement opening of the glass door The comparison measu rement must be performed when the device is completely heated up Comparison measurement procedur...

Page 70: ...value 1 0 Should deviations of more than 1 0 occur the problem must be repaired by the Technical Service only 10 6 CO2 calibration procedure 1 Activate the calibration Keep the key depressed for 5 sec...

Page 71: ...5 Unscrew sterile filter 2 from the threaded hole 3 6 When installing the new sterile filter make sure that the plastic thread is not canted Screw filter in carefully all the way to the stop 7 Instal...

Page 72: ...the outer door is located in the retaining slot No tools are required to replace the seal 1 Fig 32 Pull magnetic seal 3 out of the guide slot 1 2 Position new seal at a corner 2 and press seal retaini...

Page 73: ...rollers 50052528 Adapter plate HERAcell 150 in combination with BB 6220 or B 5060 B 5061 50066094 Adapter plate HERAcell 240 in combination with HERAcell 150 50068677 Gas tight screen split 3 elements...

Page 74: ...070316 Plug for pipe channel 50063283 IR CO2 gas tester incl charger 100 V 230 V 50060283 Spare filters set of 5 for IR CO2 gas tester 50060287 IrDa computer interface with connecting cable and PM COM...

Page 75: ...0 5 Duration of the auto start routine to 37 C ambient temperature 20 C h 5 10 Temperature recovery time at 37 C door open 30 seconds to 98 of initial value min 10 Heat transfer to environment at 37...

Page 76: ...l value Option 1 21 O2 Option 5 90 O2 min 0 5 O2 min 1 0 O2 min Retention time door open 30 seconds to 98 of initial value Option 3 O2 Option 70 O2 min 36 51 0 5 O2 min 1 0 O2 min Accuracy absolute O2...

Page 77: ...Technical data Fig 33 Overview of gas consumption CO2 Description Unit Value Rated input kW kW kW 0 60 230 VAC 0 64 120 VAC 0 62 100 VAC EMC class B Sound pressure level DIN 45 635 Part 1 dB A 50 Rela...

Page 78: ...n components Polystyrene foam EPS PPS Compound Printed circuit boards Enclosed electrical components coated with different plastics equipped on epoxy resin bound boards Plastic components general Note...

Page 79: ...fectant Do not wear protective clothing outside the work area Autoclave or disinfect contaminated devices prior to cleaning Germ contaminated waste must be collected safely and destroyed by autocla vi...

Page 80: ...ck Epidemic Act and Livestock Epidemic Germ Di rective is required Pregnant women and breast feeding mothers must not handle infectious hu manopathogenic biological agents or materials containing thes...

Page 81: ...tions 81 16 Anhang 15 Device log NOTE Device log Record nameplate information work carried out maintenance work and repairs here Part number Serial number Service number Location Operator s note Date...

Page 82: ...CO2 Incubator HERAcell 150 Operating Instructions 82 16 Certificate of decontamination...

Page 83: ...CO2 Incubator HERAcell 150 Operating Instructions 83 16 Certificate of decontamination...

Page 84: ...Internet http www kendro com...

Page 85: ......

Page 86: ...io nal sensors wires or flexible hoses into the interior of the HERAcell incubator When running electrical equipment in the interior of the HERAcell incubator the safety requirements con cerning the e...

Page 87: ...Valid 10 2005 50047292 B Gas Cylinder Change Over Unit GM 2 Operating instructions GB...

Page 88: ...les Service 0800 1 112114 E Mail info labequipment de thermo com Enquiries from North America Phone 1 800 879 7767 Fax 1 828 658 0363 E Mail info labequipment thermo com Enquiries from Europe Middle E...

Page 89: ...s contracted with one or more recycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Thermo Electron s compliance w...

Page 90: ...ially personal injury read this manual careful ly before using the equipment and follow all instructions 1 INSTRUCTIONS FOR SAFE OPERATION 3 4 Explanation of icons 3 General 4 Areas of application 4 S...

Page 91: ...ruction manual Indicates information in the instruction manual for using the equipment most effectively Gas cylinder full cylinder pressure 0 6 bar Gas cylinder empty cylinder pressure 0 6 bar Manual...

Page 92: ...ially damage to health The unit may only be operated by personnel instructed in its use When setting up and operating the unit make sure that you comply with the instructions con tained in this manual...

Page 93: ...setting of the red selector switch on the side of the unit So that the indicated setting 230 changes to 115 Gas supply connection The gas supply connections of the gas cylinder change over unit are d...

Page 94: ...ed circuits max 250 V AC max 6 A SELV SELV E circuits cf VDE 0100 Part 410 25 50 V AC 60 120 V DC max 2 1 A max 1 0 5 A Fig 2 Application example of floating contact connection Notice WARNING EXTERNAL...

Page 95: ...amp will begin flashing so that the empty cylinder can be identified After the empty cylinder has been replaced the red or flashing yellow signal lamps will turn off and the alarm output will no longe...

Page 96: ...umption W 5 Fuse supplied by customer Fuse T 16 A Power contactor G 16 6 MATERIALS USED Component Material Outer housing ABS Controls and display panel Polyethylene Other components Encapsulated elect...

Page 97: ...on manual 50 047 292 Hose set for gas supply connection 26 139 129 Supply main 230 VAC 50 043 143 Supply main 120 VAC 50 043 145 Plug for floating contact 50 034 772 Hose nozzle 26 137 409 Flexible pr...

Page 98: ...outputs are connected to a female 15 pole D Sub connector which is located at the rear of the incubator External devices can be connected to the interface by using the standart RGB S video cable whic...

Page 99: ...isconnect incubator 2 Open the HERAline interface with a cross tip srewdriver and stick the jumpers S1 according to drawing 50 053 075 3 Close the analog interface 4 Use interface cable to connect int...

Page 100: ...face of the incubator The analog outputs are connected to a female 15 pole D Sub connector which is located at the rear of the incubator External devices can be connected to the interface by using the...

Page 101: ...ect incubator 2 Open the HERAline interface with a cross tip srewdriver and stick the jumpers S1 according to drawing 50 053 075 3 Close the analog interface 4 Use interface cable to connect interface...

Page 102: ...tputs are connected to a female 15 pole D Sub connector which is located on the analog interface External devices can be connected to the interface by using the standart RGB S video cable which comman...

Page 103: ...ct incubator 2 Open the HERAline interface with a cross tip srewdriver and stick the jumpers S1 according to drawing 50 053 075 3 Close the analog interface 4 Use interface cable to connect interface...

Page 104: ...ce of the incubator The analog outputs are connected to a female 15 pole D Sub connector which is located on the analog interface External devices can be connected to the interface by using the standa...

Page 105: ...ubator 2 Open the HERAline interface with a cross tip srewdriver and stick the jumpers S1 according to drawing 50 053 075 3 Close the analog interface 4 Use interface cable to connect interface of the...

Page 106: ......

Page 107: ...CTRONIC CONCEPT 4 4 3 DESCRIPTION OF FUNCTIONAL GROUPS 7 4 4 SWITCHING THE UNIT ON 11 4 5 CONTROL AND REGULATING SYSTEMS CONFIGURATION 12 4 6 ERROR TABLE 20 4 7 ERROR EXAMINATION AND REGULATION CIRCUI...

Page 108: ...t soweit nicht ausdr cklich zugestanden Zuwiderhandlungen ver pflichten zu Schadensersatz Alle Rechte f r den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten Kendro Laboratory Prod...

Page 109: ...erteilung oder Gebrauchsmustereintragung vorbehalten Kendro Laboratory Products GmbH Germany 4 1 DESCRIPTION OF CONTROL AND REGULATING SYSTEM HERAcell unit is equipped with the following measurement c...

Page 110: ...asurement tasks on site Thus one P controls the display on the unit door while another one on the sen sor board controls the fan and generates measured values for temperature and CO2 only if the therm...

Page 111: ...Dokumentnr Document No Seite Page Datei File 50051109_04_EN_C doc 50051109 C 5 9 Weitergabe sowie Vervielf ltigung dieser Unterlage Verwertung oder Mitteilung ihres Inhalts nicht gestattet soweit nich...

Page 112: ...r Document No Seite Page Datei File 50051109_04_EN_C doc 50051109 C 6 9 Weitergabe sowie Vervielf ltigung dieser Unterlage Verwertung oder Mitteilung ihres Inhalts nicht gestattet soweit nicht ausdr c...

Page 113: ...the master processor The signal is compared with the PT1000 value to serve as a plausibility check of the measured temperatures If the difference between the two measured temperatures exceeds a range...

Page 114: ...age for the electronic components Voltage is displayed by an LED Power for the auto zero air pump is derived from the main PCB s power supply Signal processing water level sensor Temperature and CO2 c...

Page 115: ...232 interface An RS 232 interface is part of the standard unit equipment Control of the zero potential alarm contact In case of a fault the standard alarm contact center zero relay is activated Door...

Page 116: ...the device description The basic device configuration parameters are stored on functional levels refer to the control and regulating system configuration description All essential adjustment routines...

Page 117: ...he main power control indicates that power to the unit has been turned on Both displays perform an 8 digit test and all LEDs come on When high low humidity O2 and or flask rotation equipment are confi...

Page 118: ...FL 4 described below are only used to monitor the val ues entered here Normally these values should only be changed by the fac tory adjustment Any required device calibrations can be performed with t...

Page 119: ...omatically determined The settings may only be modified after consultation with the factory Levels 1 Adjustments for regulating range 1 incubation mode 0 55 C Sublevel CO2 display Sublevel value range...

Page 120: ...ntamination temperature offset value in C 3 0 1 Decontamination temperature amplifica tion pre decimal places 4 000 999 Decontamination temperature amplifica tion decimal places 5 55 90 Decontaminatio...

Page 121: ...zero point calibration 4 0 1 Manual auto zero activation 5 0 99 9 Display of LM 75 without correction 6 19 9 19 9 Display LM 75 correction offset The CO2 metering bridge countervoltage is automaticall...

Page 122: ...iption 1 PH0 PH1 Door heating factor OFF Door heating factor ON 2 0 0 3 0 Door heating factor Factory setting Door factor ON PH1 May not be changed When replacing the main board the door heating facto...

Page 123: ...value NV RAM on the sensor board The decimal places are displayed 3 000 999 N2 CO2 correction value NV RAM on the sensor board The decimal places are displayed 4 0 1 1 Starts the determination of the...

Page 124: ...14 Gas guard CO2 settings FL position FL value range Description 1 0 1 Gas guard not configured configured 2 0 1 Display bottle 1 pressure low o K 3 0 1 Display bottle 2 pressure low o K 4 0 1 Display...

Page 125: ...ize 2 0 VA 1 CU Configure interior fittings 3 0 230 Volt 1 120 Volt Configure mains voltage 4 0 Not installed 1 Installed Configure gas diaphragm 5 0 No O2 1 5 90 O2 2 1 21 O2 Configure O2 6 0 Not ins...

Page 126: ...Germany Determining the unit version The following table can be used to determine the unit version Values determining the unit version Version 0 Heracell 150 1 Heracell 240 Unit size 0 VA 2 CU Interi...

Page 127: ...Main board NVRAM read er ror The mirrored values were loaded 44 NVRAM defect Values of the measuring cell are not overwrit ten unit runs using default values 54 Set value error Error in the calculati...

Page 128: ...tual value set value 1 0 CO2 204 CO2 measuring cell defective Sensor break or short circuit or infrared measuring cell defective 205 Humidity sensor probe break Humidity sensor in the sensor block def...

Page 129: ...ose that cannot be assigned to a specific regulating circuit Error code Test equip ment Test at the unit Inspections and tests Possible corrective actions Multimeter Check the power supply to the O2 b...

Page 130: ...s power supply unit 55 Bus cable and sensor block both in working order Switch the unit off Disconnect the bus ca ble to the sensor block at the main board Attach the test set ca ble sensor block Swit...

Page 131: ...Sample chamber with controlled directly mounted heating lines The unit s air jacket supports the temperature equilibrium Exterior door with mounted heating lines to prevent condensation from forming...

Page 132: ...pections and tests Possible corrective ac tions 66 Bus cable and sensor block both in working order Switch the unit off Attach the test set ca ble sensor block Switch the unit on Perform the functiona...

Page 133: ...temperature Decontamination phase Duration 9 hours Once the unit reaches the lower limit band value 85 C the de contamination time holding counter starts Condensation phase Duration 6 hours The unit s...

Page 134: ...r Patenterteilung oder Gebrauchsmustereintragung vorbehalten Kendro Laboratory Products GmbH Germany Decontamination status You can also output the precise phase of the decontamination routine that th...

Page 135: ...ersatz Alle Rechte f r den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten Kendro Laboratory Products GmbH Germany 4 7 5 ERROR IN THE TEMPERATURE MEASUREMENT REGULATING CIRCUIT DEC...

Page 136: ...based CO2 gas input is metered by a solenoid The gas flowrate is also restricted by a capillary opening with a diameter of 0 7 mm integrated in the valve block Before entering the unit the gas passes...

Page 137: ...s sure Check the flow through the sterile filter Perform the solenoid func tional test FL 3 Check the flow through the solenoid Check the tubing to the de tector Check the air pump Check the auto zero...

Page 138: ...le plugged in sensor head auto cal routine for automatic adjustment is integrated into the auto start routine P based O2 regulator O2 or N2 input via the same connection spout After entering the sampl...

Page 139: ...lay Test equip ment Test at the unit Inspections and tests Possible corrective ac tions 300 O2 Gas on LED continuously lit Check the initial gas pres sure Check the flow through the sterile filter Per...

Page 140: ...er Check the sample chamber heater ac tuators heaters E2 E8 Check the unit ver sion Replace the main board Return to factory for repairs Set correct set of parameters FL 601 Heavy condensa tion on the...

Page 141: ...ucts GmbH Germany 4 8 FAN SYSTEM AND ERRORS Short description DC motor nominal voltage 24 V operating voltage 12 V mounted on the sensor block Located outside the sample chamber The fan speed is adjus...

Page 142: ...ror info If the pressure in both cylinders is below 0 6 bar an alarm will sound the potential free contact will switch and an error info on the display CO2 or O2 will flash Errors ot the gaws guard Er...

Page 143: ...functional tests and control measurements have been performed the unit MUST be restarted with the auto start routine Units without O2 The replacement sensor block has been fully preadjusted and calibr...

Page 144: ...4 3 Remove the gas from the unit and restart the unit with the auto start routine Unit with IR measuring cell Se Section 4 14 5 4 12 MAIN BOARD REPLACEMENT After completing the service tasks After th...

Page 145: ...chadensersatz Alle Rechte f r den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten Kendro Laboratory Products GmbH Germany the zero point adjustment of the CO2 metering circuit Run...

Page 146: ...outine can be initiated for each adjustable regulation circuit by using the cal key 4 14 1 TEMPERATURE ADJUSTMENT INCUBATION MODE Comparative meas urement Incubation temperature Perform the comparativ...

Page 147: ...ve measurement Perform the comparative measurement during the decontamination phase of the de contamination routine Allow the unit to reach equilibrium before taking the reading For cold units No soon...

Page 148: ...l a comparative measurement should be made unless the actual value is known e g CO2 display deviation at 0 0 CO2 Adjustment routine activation Refer to the example in the operating instructions Chapte...

Page 149: ...g oder Gebrauchsmustereintragung vorbehalten Kendro Laboratory Products GmbH Germany Checking and Adjusting the O2 Compensation at the TCD Measuring Cell Preparation Remove the CO2 from the unit Adjus...

Page 150: ...asurements at the following O2 concentrations Sensor measurement range 1 21 O2 approx 5 O2 Sensor measurement range 5 90 O2 approx 90 O2 Give the unit sufficient time to stabilize before reading the m...

Page 151: ...ting the sensor a reference device must be employed to ensure that the device setup area is not being artificially enriched with CO2 as a result of inadequate ventilation Zero point calibration CO2 co...

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Page 176: ...very slowly CO2 tube and or metering cable wires are lying against the fan motor Disassemble the measuring cell Check the position of the CO2 tube in the guide groove When reinstalling the cell make s...

Page 177: ...calibrating the sensor a reference device must be employed to ensure that the device setup area is not being artificially enriched with CO2 as a result of inadequate ventilation Zero point calibration...

Page 178: ...43 Main board NVRAM read e r ror The mirrored values were loaded 44 NVRAM defect Values of the measuring cell are not overwri t ten unit runs using default values 54 Set value error Error in the calc...

Page 179: ...LM 75 defective Probe break or sensor short circuit 200 CO2 below set value Actual value set value 1 0 CO 2 201 CO2 above set value Actual value set value 1 0 CO 2 204 CO2 measuring cell defective Se...

Page 180: ...e those that cannot be assigned to a specific regulating circuit Error code Test equip ment Test at the unit Inspections and tests Possible corrective actions Multimeter Check the power supply to the...

Page 181: ...ains power supply unit 55 Bus cable and sensor block both in working order Switch the unit off Disconnect the bus ca ble to the sensor block at the main board Attach the test set ca ble sensor block S...

Page 182: ...Assembly Instruction Lockable door for HERAcell HERAcell 150 and HERAcell 240 Edition 09 2003 50072431 A...

Page 183: ...fitting the CO2 incubators HERAcell HERAcell 150 and HERAcell 240 This door lock can be retrofitted to all CO2 incubators HERAcell HERAcell 150 and HERAcell 240 The device must be removed from service...

Page 184: ...cell 240 3 4 Retrofit door lock 4 1 HERAcell HERAcell 150 and HERAcell 240 non stacked devices 1 Unscrew the front stacking element of the unit 2 Remove the adjacent cap of the outer door 3 Mount the...

Page 185: ...1 Place the drilling jig flush with the top of the outer casing 2 Mark the position of the three holes with the marker pen 3 Before drilling the holes use a centre punch to mark the position 4 Drill t...

Page 186: ...oor for HERAcell HERAcell 150 and HERAcell 240 5 5 Open up the hole in the door first with a 6 mm drill and then with an 8 mm 6 Holes for the door lock 7 Mount the door lock with the screws and washer...

Page 187: ...the drilling jig on the upper edge of the power switch housing 2 Mark the position of the three holes with the marker pen 3 Before drilling the holes use a centre punch to mark the position 4 Drill th...

Page 188: ...oor for HERAcell HERAcell 150 and HERAcell 240 7 5 Open up the hole in the door first with a 6 mm drill and then with an 8 mm 6 Holes for the door lock 7 Mount the door lock with the screws and washer...

Page 189: ...he drilling jig flush with the top of the door handle 2 Mark the position of the three holes with the marker pen 3 Before drilling the holes use a centre punch to mark the position 4 Drill the marked...

Page 190: ...cell HERAcell 150 and HERAcell 240 9 5 Open up the hole in the door first with a 6 mm drill and then with an 8 mm 6 Counter sink the hole 7 Holes for the door lock 8 Mount the door lock with the screw...

Page 191: ...e drilling jig flush with the bottom of the door handle 2 Mark the position of the three holes with the marker pen 3 Before drilling the holes use a centre punch to mark the position 4 Drill the marke...

Page 192: ...door lock with the screws and washers provided Address Kendro Laboratory Products GmbH Robert Bosch Strasse 1 D 63505 Langenselbold Telefone Sales Service 49 0 6184 90 6940 Fax Sales Service 49 0 618...

Page 193: ...und stecken jetzt den Schlauch auf die T lle der Gaszuf hrung Bild 2 3 Then place the gas humidifier assembly in the chamber rear right parallel to the rear wall and connect the tube to the gassupply...

Page 194: ...Installation Instructions Gas Tight Screen for the HERAcell and HERAcell 150 Retrofit Version 07 2003 50078424...

Page 195: ...tion These installation instructions describe the prozess for retrofitting the HERAcell and HERAcell 150 CO2 incubators with a gas tight screen List of Required Tools Knife Allen key Gr e 3 mm Phillip...

Page 196: ...cell 150 Retrofit Version 07 2003 50078424 3 2 Device Preparation 1 The device must be taken out of action to perform the retrofit work 2 The device should be standing on a firm base Glass door upper...

Page 197: ...pry off the cover panels on the upper and lower door hinges 3 Remove the Allen screw 3 mm on the upper door hinge The glass door shouls still be closed during this step 4 Open the glass door and remo...

Page 198: ...lation 1 Insert the gas tight screen with the 2 washers into the door hinge Close the gas tight screen 2 Now insert the upper door hinge onto the gas tight screen shaft Secure the door hinge with the...

Page 199: ...rivets from the inside of the door 1 1 Using a thin nail puncture the center of the rivets inwards 1 2 Using the kife CARREFULLY pull the rivets out 2 Using the Phillip s head screwdriver mount the a...

Page 200: ...or the HERAcell and HERAcell 150 Retrofit Version 07 2003 50078424 7 3 Using Phillip s head scres mount the shackle serves as switch activator on the inside of the exterior door 4 Clip the cover panel...

Page 201: ...of the holes must be observed For that purpose in a distance of 86 mm to the upper door edge a horizontal mark must be sketched Please measure the middle of the door On each site of the middle left an...

Page 202: ...Gas Tight Screen for the HERAcell and HERAcell 150 Retrofit Version 07 2003 50078424 9 4 Using Phillip s head scres mount the shackle serves as switch activator on the inside of the exterior door...

Page 203: ...nd the auto start key and hold them down for at least 5 seconds The programm enters function level 0 2 To move to the various function levels press and hold down the cal key and use the key Go to func...

Page 204: ...240 230 V Cu 7 HERAcell 240 120 V VA 8 HERAcell 240 120 V Cu 9 10 11 12 HERAcell 240 230 V VA HERAcell 240 230 V Cu HERAcell 240 120 V VA HERAcell 240 120 V Cu Set of parameters for gas tight screen b...

Page 205: ...ey the i key and the auto start key and hold them down for at least 5 seconds The programm enters function level 0 2 To move to the various function levels press and hold down the cal key and use the...

Page 206: ...ng device 7 0 standart TCD or IR 1 Auto Zero measuring cell Configure installed measuring cell 8 0 not installed 1 installed Configure water level sensor 9 0 inactive 1 active Configure low humidity o...

Page 207: ...Acell 150 Retrofit Version 07 2003 50078424 14 Anschrift Kendro Laboratory Products GmbH Robert Bosch Stra e 1 D 63505 Langenselbold Deutschland Telefon Vertrieb 49 0 1805 536376 Service 49 0 1805 112...

Page 208: ...Assembly Instructions HERAcell HERAcell 150 and HERAcell 240 Main PCB Replacement Version 12 2003 50073246 D...

Page 209: ...4 2 2 Rear View units with options or with large control box 5 3 Main PCB Replacement 6 3 1 Disconnecting the Device from the Mains Power Supply 6 3 2 Removing the Main PCB 6 3 3 Installing the New P...

Page 210: ...nly be performed by Kendro Service or specialist personnel author ized by Kendro Should retooling be performed by anyone other than authorized service personnel all warranties provided by Kendro Labor...

Page 211: ...ositioned on a stable base table bench 2 1 Rear View units without any options small control box 1 Mains power plug 2 Alarm output 3 Mains power circuit orange connector 4 Terminal door switch 5 Bus c...

Page 212: ...wer plug 2 Alarm output 3 Mains power circuit orange connector 4 Terminal for door switch 5 Bus cables max 3 pcs 6 Terminal for CO2 solenoid valve 7 Ground connection 8 Terminal for water level sensor...

Page 213: ...g terminals with a small screwdriver 7 Unplug the bus cables at the plug in connections JP3 JP4 JP 15 Press the lockinng hook on the connectors to release 8 Disconnect the two CO2 solenoid leads from...

Page 214: ...O2 Next select the appropriate parameter set for your unit from the table below Device assignment to the control parameter sets Parameter set no Device Note 1 HERAcell 230 V SS SS stainless steel 2 HE...

Page 215: ...e the settings and initiate a device reset 8 digit check of both displays and all LEDs are on If High Low moisture has been configured the associated LED will also light up during the 8 digit check Th...

Page 216: ...then displays the current actual values of temperature and CO2 The parameter number will displayed during initialization after displaying the software version P1 P2 P3 The display will show Pn If the...

Page 217: ...le turners 7 0 standard TCD or IR 1 Auto Zero detector CO2 detector 8 0 not installed 1 installed Water Level Sensor 9 0 inactive 1 active High Low Humidity 10 0 not installed 1 installed Gas guard CO...

Page 218: ...the values must be stored to the memory as follows Press the cal key several times an keep it pressed after reaching sublevel 20 During pressing the cal key use the key and select 1 Releasing the cal...

Page 219: ...HERAcell 240 Main PCB Replacement 12 Address Kendro Laboratory Products GmbH Robert Bosch Str 1 D 63505 Langenselbold Germany Telephone Sales Service 49 0 6184 90 6940 Fax Sales Service 49 0 6184 90...

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Page 232: ...30 V right X 50077894 Main door HERA 150 for 230 V left Ger tet r HERAcell 150 230 V links X 50077895 Main door HERA 150 for 120 V right Ger tet r HERAcell 150 120 V rechts X 50077896 Main door HERA 1...

Page 233: ...120 V X 50077197 Door panel heated for 120 VAC units T rblech beheizt HERAcell 120 V X 50069358 Door panel HERA 240 heated 230 VAC units T rblech beheizt HERAcell 240 230 V X X 50069359 Door panel HER...

Page 234: ...le GLASTUER VST GASBLENDE HERACELL 150 OL X 50077913 Glass door for Gas screen HERAcell 150 with hole GLASTUER VST GASBLENDE HERACELL 150 ML X 50077046 Seal 3 Door screen T rdichtung gasblendet r X 50...

Page 235: ...be 4 x 3 mm Schlauch 4 x 3 X X X X 03719098 Y connector for tube distribution Di 4 mm Y verbinder YS 4 Di 4 mm X X X X 50062978 Straight tube connector f r Di 4 mm Schlauchverbinder GS 4 di 4 mm X X X...

Page 236: ...w cut the seal to the correct length Make sure that the seal is cut at right angles 2 Remove the old seal 3 Fit the new seal Ersatzteil Glast rdichtung f r CO2 Inkubatoren Spare part Glass door seal f...

Page 237: ...Version 04 2004 50078754 Accessories for CO2 incubators Illustrations and describtion...

Page 238: ...ies for HERAcell HERAcell 150 3 1 1 Support frames 3 1 2 Shelves HERAtrays 4 1 3 Stack adapters 4 2 Accessories for HERAcell 240 5 2 1 Support frames 5 2 2 Shelves HERAtrays 6 2 3 Stack adapters 7 3 A...

Page 239: ...2 Support frame 780 mm Width 637 mm Height 780 mm Depth 600 mm 50 051 436 3 Support frame 185 mm with castors Width 637 mm Height 185 mm Depth 600 mm 50 057 161 4 Support cart with drawers and castors...

Page 240: ...0 051 914 3 HERAtray 1 2 width SS 2 pcs HERAtray 1 2 width Cu 2 pcs Width 205 mm Overall Depth 440 mm Overall Thickness 1 mm 50 058 672 50 061 050 1 3 Stack adapters 1 Plate for stacking a HERAcell or...

Page 241: ...mm Height 200 mm Depth 652 mm 50 065 754 2 Support frame 780 mm Width 780 mm Height 780 mm Depth 652 mm 50 065 753 3 Support frame 185 mm with castors Width 780 mm Height 185 mm Depth 652 mm 50 067 22...

Page 242: ...ERAtray 1 4 width SS 3 pcs HERAtray 1 4 Width Cu 3 pcs Width 135 mm Overall Depth 485 mm Overall Thickness 1 mm 50 065 807 50 065 808 4 HERAtray 1 3 width SS 3 pcs HERAtray 1 3 Width Cu 3 pcs Width 18...

Page 243: ...240 or for stacking a HERAcell or HERAcell 150 with a HERAcell 240 Width 780 mm Depth 772 mm 50 068 677 2 Plate for stacking a HERAcell 240 with a BB 6220 or BBD 6220 or cytoperm 2 or B 5060 or B 506...

Page 244: ...upport frame 780 mm Width 885 mm Height 780 mm Depth 645 mm 50 029 597 3 Stacking frame with castors for stacking 2 x BBD 6220 or cytoperm 2 Support frame for lower unit Width 920 mm Height 200 mm Dep...

Page 245: ...Accessories for CO2 incubators Version 04 2004 50078754 9 4 Castors set for 1 2 above Set of 4 Information When fitting the castors will increase the hight of the support frame by 90 mm 50 052 528...

Page 246: ...4 width SS 3 pcs Width 135 mm Overall Depth 485 mm Overall Thickness 1 mm 50 065 807 4 HERAtray 1 3 width Cu 3 pcs Width 180 mm Overall Depth 485 mm Overall Thickness 1 mm 50 065 805 5 Shelf for unit...

Page 247: ...2004 50078754 11 3 3 Stack adapter 1 Plate for stacking a HERAcell 240 with a BB 6220 or BBD 6220 or cytoperm 2 or B 5060 or B 5061 Width 895 mm Depth 721 mm Information The BBD 6220 or cytoperm 2 sh...

Page 248: ...2 incubators Version 04 2004 50078754 12 Anschrift Kendro Laboratory Products GmbH Robert Bosch Stra e 1 D 63505 Langenselbold Deutschland Telefon Vertrieb Service 49 0 6184 906940 Fax Vertrieb Servic...

Page 249: ...Footprint HERAcell...

Page 250: ...Footprint HERAcell...

Page 251: ...NFORMATION Due to poor reliability 50075590 water level sensor for HERAcell has been replaced with a new more robust device The spare part number for the new sensor is 50084764 and is available immedi...

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