Installing the Nicolet iN5 Microscope
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1/4/2018
Page 70 of 84
j. Using a .05 inch balldriver, loosen the rotational [focus] screw on the
ATR slide enough to rotate the crystal.
k. From the Collect drop-down menu select collect sample, select the
default spectrum title or enter a title, click OK and leave the sample
collection in preview mode.
l. Rotate the crystal for maximum throughput (see bullet points below)
and when complete, secure the rotational [focus] screw.
Note
Observe the throughput while securing the rotational screw. If
throughput degrades, loosen the setscrew and iterate between rotation and
securing the setscrew to maintain maximum throughput.
•
When rotating the crystal, do not push up on the crystal; use your finger at
the edge crystal mount to rotate.
•
Throughput using a sample aperture size of 100 x 100µm should be equal
or better than 30% of reflectance at 4000 wavenumbers. Refer to the
following page.
