4 • System Plumbing
Doc. 065447-02 12/14
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3. Remove the
EG DEGAS IN
and
EG DEGAS OUT
lines from the pre-
assembled tubing chain (see
) and connect them to their
respective ports on the EG degas cartridge.
4. Connect the EG degas
ELUENT OUT
line to the injection valve
ELUENT IN
port.
5. Set the pump
Purge
rate to 0.1 mL/min and the
Duration
to 1800 s. Click
Prime
. Do not open the purge valve (you can cancel the Ready Check
message that appears). The regenerant path is flushed for 30 minutes.
NOTE
The pump pressure is typically 3 to 5 MPa (400 to
700 psi) at 0.100 mL/min.
6. Monitor the waste flow from the EG degas. When no bubbles are present,
the path is completely flushed and filled.
7. Check the backpressure to confirm that the liquid path is ready:
a.
Note the pump pressure.
b. Disconnect the Suppressor Bypass
REGEN IN
from the ED cell
OUT
(the
high-pressure side) and note the difference in the pump pressure.
c.
If the difference is <0.7 MPa (100 psi), the low-pressure flow path is
ready and you can proceed to the next step.
NOTE
If the pressure difference is greater than 0.7 MPa (100 psi),
refer to the ICS-5000
+
operator’s manual for troubleshooting
information on high system backpressure.
4.1.10 Flushing and Conditioning the Electrolytic Devices
Preparing the Dionex EGC
1. Verify that the vent fitting is tightened. This prevents cartridge leaks
during installation.
2. While holding the Dionex EGC with the ports on the bottom, shake the
cartridge vigorously and tap it with the palm of your hand 10 to 15 times
to dislodge air bubbles that may be trapped in the chamber.
3. Remove the fitting plugs from the
EGC IN
and
EGC OUT
ports.
Capillary
IC