Molecular Devices AquaMax 2000, Руководство пользователя

Страница: 115 / 158

0112-0151 K 115
C
AquaMax Sterilant
AquaMax
®
Sterilant (Part Number: R8156) can be used to clean and
sterilize the fluid paths in the AquaMax Microplate Washer.
Principle of Use
Accumulated residues and contaminating microorganisms can impair the
operation of liquid handling equipment. Proteins in cell culture media,
salts and minerals in buffers, and other reagent components can build up
on fluid path surfaces. This can clog channels and probes and can result
in incomplete or inaccurate dispensing and aspiration. Microorganisms
such as bacteria, fungi, and molds can accumulate in the fluid path
creating similar fouling problems. Simply flushing the fluid path with
water or ethanol/water solutions is often ineffective in removing residue.
AquaMax Sterilant was developed to remove organic and inorganic
material and kill microorganisms, including endospores, in fluid paths in
liquid handling instruments.
The AquaMax Sterilant simultaneously removes endotoxin from the fluid
paths. Endotoxin is a substance produced by Gram negative bacteria that
can cause a variety of significant cellular reactions in vitro, including cell
activation and death. Minimizing the amount of endotoxin in fluid paths
is necessary to avoid detachment and adverse effects on mammalian
cells during the course of subsequent experiments. A two-hour
treatment with the AquaMax Sterilant will substantially remove
endotoxin adhering to interior fluid path surfaces.