Waters Xevo TQD, Overview And Maintenance Manual

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3 Changing the Mode of Operation
80 March 14, 2014, 715004387 Rev. B
15. Carefully push the stage back into the NanoFlow source enclosure, using
the stop and handle.
Positioning the borosilicate glass capillary tip
Having obtained a signal, you must adjust the tip position to maximize it.
Using the three-axis manipulator, you can adjust the tip position up and
down, left and right, forward and backward. As a starting point, set the tip so
that it is on the center line of the sampling cone and at a distance between two
and three times the diameter of the cone aperture. Typically this distance is
approximately 2 mm.
Capillary tip position:
For tuning instructions, see the instrument’s online help.
Restarting a stalled borosilicate glass capillary electrospray
Should the spray stop, you can restart it. To do so, in the MassLynx Tune
window, set Capillary to 0 kV. Then adjust the three-axis manipulator so that,
viewed under magnification, the capillary tip touches the sample cone, and a
small piece of the borosilicate glass capillary shears off.
If necessary, you can also apply some NanoFlow gas pressure, to force a drop
of liquid from the capillary. Apply as much as 1.4 bar (20 psi). Doing so
induces the drop’s appearance unless the capillary is blocked.
Notice:
To prevent the capillary tip from colliding with the cone or
the side of the source, adjust the sprayer tip position before you
push the sprayer inside the NanoFlow source enclosure.
d
2d
3d
Cone aperture diameter