Waters 2489, Overview And Maintenance Manual

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June 11, 2015, 715004752 Rev. A
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3.2.4 Primary and secondary functions ....................................................... 57
3.2.5 Operating other detector functions..................................................... 63
3.2.6 Operating the detector ..................................................................... 66
3.2.7 Verifying that the detector operates properly....................................... 67
3.2.8 Wavelength calibration ..................................................................... 69
3.2.9 Operating the detector in single-wavelength mode ............................... 69
3.2.10 Operating the detector in dual-wavelength mode ............................... 70
3.3 Scanning spectra ...................................................................................... 78
3.3.1 Before you begin ............................................................................. 78
3.3.2 Scanning new spectra ...................................................................... 80
3.3.3 Storing a spectrum .......................................................................... 86
3.3.4 Reviewing a stored spectrum ............................................................ 87
3.3.5 Subtracting a spectrum .................................................................... 87
3.3.6 Replaying a spectrum....................................................................... 88
3.3.7 Scanning using the cuvette ............................................................... 88
3.3.8 Scanning using a flow cell and a syringe ............................................. 90
3.3.9 Conserving lamp life ........................................................................ 90
3.3.10 Shutting down the detector ............................................................. 92
4 Maintaining the Detector ......................................................... 93
4.1 Contacting Waters technical service ............................................................. 93
4.2 Maintenance considerations ........................................................................ 93
4.2.1 Safety and handling ......................................................................... 94
4.2.2 Spare parts .................................................................................... 94
4.3 Proper operating procedures ...................................................................... 94
4.3.1 Routine maintenance ....................................................................... 94
4.4 Maintaining the flow cell ............................................................................ 95
4.4.1 Flushing the flow cell ....................................................................... 95
4.4.2 Removing and cleaning the flow cell ................................................... 97
4.4.3 Disassembling and reassembling the flow cell...................................... 98
4.5 Replacing the lamp ................................................................................. 103
4.5.1 Lamp characteristics ...................................................................... 104
4.5.2 Lamp energy and performance ........................................................ 104
4.5.3 Removing the lamp........................................................................ 105
4.5.4 Installing the new lamp .................................................................. 107