June 11, 2015, 715004752 Rev. A
Page 21
Figure 1–4:
Filter time constant comparison
Tip:
Although the peak shape shows some distortion and the signal output is delayed
with different time constants, the peak area remains the same.
1.2.2 Wavelength verification and test
The detector’s deuterium arc lamp and integral erbium filter exhibit peaks in the
transmission spectrum at known wavelengths. Upon startup, the detector verifies
calibration by comparing the locations of these peaks with expected wavelengths
based on calibration data stored in the detector’s memory. If the results of this
verification differ from the stored calibration by more than 1.0 nm, the detector
displays a Wavelength Verification Failure message. The detector verifies, rather than
recalibrates, on startup to avoid errors arising from residual materials left in the flow
cell or the cuvette, when required.
Requirement:
If your flow cell requires a cuvette, always ensure that the cuvette is
removed and the cuvette holder and front door are secured during startup
verification.
You can initiate a manual wavelength calibration at any time. A manual calibration
replaces the previous calibration data with new data.
“Wavelength calibration” on page 69
for the manual wavelength calibration
procedure.
The verification and calibration algorithms are virtually identical. However, the
verification algorithm can issue an error message indicating that actual data do not
match stored data where the calibration algorithm replaces the stored data with the
new.
0 sec
1 sec
2 sec
Time (minutes)
Absorbance
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