Waters 2489, Overview And Maintenance Manual

Page: 89 / 150

June 11, 2015, 715004752 Rev. A
Page 89
The detector uses a standard, 10-mm path length, spectrophotometric cell (quartz
cuvette). You insert the cuvette with one of the two frosted sides facing up in the
cuvette holder and place it in the detector flow cell assembly.
Figure 3–33: Detector cuvette holder with the cuvette inserted
Restriction:
Because the scan is actually a composite of both the contents of the
cuvette and the flow cell, you need to perform cuvette scans under identical flow cell
conditions. If you store spectra and acquire new spectra for subtraction, beware the
differences, if any, in flow cell conditions.
Ideally, you perform both the zero and sample scans using the cuvette when the HPLC
instruments are in the idle or static state, under identical flow cell conditions.
3.3.7.1 Before you begin
Recommendation:
To ensure accurate results, use a 10-mm path length, quartz
cuvette and matched pairs (from the same manufacturing lot) of quartz cuvettes for
your zero and sample scans.
Before beginning a scan using the cuvette:
1. Flush the flow cell with the eluent you are going to scan.
2. Wipe the clear portion of the cuvette with low-lint, nonabrasive tissue wipes.
To begin a cuvette scan:
1. Open the detector’s front door.
2. Remove the cuvette holder, sliding it toward you.
3. With the spring guide facing you, gently insert the cuvette (containing eluent)
up and under the guide, with the cap facing upward (into the holder) and a
frosted side of the cuvette facing up. (See the figure on page 89 .)
Notice:
To avoid compromising the integrity of cuvette scanning operations,
handle the cuvette gently and on the frosted sides only. Fingerprints on the
clear quartz interfere with the light path.
Aperture
Frosted sides of cuvette
facing up and down