June 11, 2015, 715004752 Rev. A
Page 17
1.2 Principles of operation
To use the detector effectively, become familiar with its optical and electronic design
and the theory and principles of its operation.
This section describes the following parts and functions of the detector:
•
Optics
•
Wavelength verification and test
•
Flow cell
•
Electronics
1.2.1 Detector optics
The 2489 UV/Vis detector optics are based on a Fastie-Ebert monochromator and
include these components:
•
High brightness deuterium (D
2
) lamp
•
Two mirrors: one off-axis, ellipsoidal mirror and one spherical mirror
•
Filter wheel
•
Shutter, wavelength calibration filter, and second-order filter
•
Entrance slit
•
Blazed, plane-holographic, diffraction grating
•
Beamsplitter
•
Sample and reference photodiodes
•
Waters TaperSlit™ Flow Cell (its entrance is the exit slit of the monochromator)
•
Cuvette holder for flow cells requiring a cuvette.
If your flow cell requires a cuvette:
Cuvette qualification
Facilitates qualification of the detector by
insertion of a standard in a cuvette
without breaking any plumbing
connections. Waters qualification kits,
available in cuvette form, support this
feature, which allows the detector to
serve as a benchtop spectrophotometer.
Cuvette sample analysis
Allows recording of the spectrum of any
sample placed in the cuvette.
Table 1–1:
Detector capabilities
Capability
Description
Summary of Contents for 2489
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