June 11, 2015, 715004752 Rev. A
Page 67
3.2.6.4
Remote control
To operate under Empower or MassLynx control, the detector uses the Ethernet bus
connector (see
).
To connect the detector to your HPLC system, see
.
When under the control of an external data system, the detector is operating under
remote control conditions. The Remote Control icon, incorporating the letter “E”,
appears on the absorbance screen (see the table on
for more information on connecting the detector to an external system.
3.2.7 Verifying that the detector operates properly
After you have installed the detector, verify that it is operating properly by performing
the procedures in this section.
Note:
If your flow cell does not require a cuvette, you can obtain an analytical flow
cell to perform the validation procedures. See the Waters Quality Parts Locator on the
Services and Support page of the Waters’ web site.
Requirements:
•
For complete validation procedures, you must obtain the Waters accuracy and
linearity cuvette kits and system qualification tool for the detector.
•
For spare parts details, see the Waters Quality Parts Locator on the Services &
Support page of the Waters’ web site.
Tip:
Before you pump solvent or mobile phase through the system:
1. Flush the lines with filtered, degassed, and sparged HPLC-grade methanol.
2. Pump your mobile phase, provided that there are no miscibility problems, at 1
mL/min for 15 minutes minimum.
3.2.7.1
Before you begin
Because the detector is shipped dry, you must pump fresh, filtered and degassed
clear solvent through the unit before its initial use.
Requirement:
To ensure accurate verification, be sure to start up the detector and
follow steps 1 to 4 in this section and 1 to 5 in
“Recording sample and reference beam
before pumping any mobile phase or solvent through the flow
cell.
To start up the instrument:
1. Connect the detector to a data system or integrator (see
for complete
information on connecting the detector to external devices).
2. Power-on the detector.
Result:
The front panel displays a series of initialization messages for about 5
minutes. See
3. When initialization ends, the detector displays the absorbance screen.
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