June 11, 2015, 715004752 Rev. A
Page 88
Tip:
The current spectrum is the spectrum that the stored spectrum (whose slot
number you enter) is subtracted from.
To subtract and review the difference spectrum:
Rule:
To subtract the stored spectrum from the current spectrum, the starting and
ending wavelengths (
λ
1 and
λ
2
)
and the pace of both spectra must be identical.
1. Press SCAN (Shift Chart-mark).
2. Press 5, Subtract & review.
3. Enter the storage slot number (1 to 3) of the spectrum you want to subtract
from the current (or retrieved) spectrum.
4. Press Enter.
Once the difference spectrum appears, you can store the results in one of the three
storage slots.
3.3.6 Replaying a spectrum
You can play back the current spectrum or a stored spectrum, in real time, using the
Real-time replay function on the Scan choice list. The detector plays back the selected
spectrum in real time both on the detector display and out the analog connector to
the chart or A/D device in a data collection system. Once you retrieve a spectrum for
playback, the detector displays it graphically, and you can adjust the AUFS. Sample
energy is not charted during a replay.
Tip:
If you adjust the AUFS on a spectrum for replay, the adjusted spectrum displays
only on the charted output, not on the detector’s graphical display.
To replay a spectrum:
1. Press SCAN (Shift Chart-mark).
2. Press 6, Real-time replay.
3. Enter the storage slot number (1 to 3) of the spectrum you want to play back.
Tip:
The default is the last spectrum acquired.
4. Press Enter.
Result:
After a pause, to retrieve the selected spectrum, the detector begins to
play back the spectrum on the analog connection. Then the spectrum appears
graphically.
3.3.7 Scanning using the cuvette
Note:
If your flow cell does not require a cuvette, you can obtain an analytical flow
cell to perform the validation procedures. See the Waters Quality Parts Locator on the
Services and Support page of the Waters’ Web site.
Using the cuvette option facilitates sample handling and verifying and qualifying the
instrument.
Summary of Contents for 2489
Page 2: ...June 11 2015 715004752 Rev A Page ii...
Page 8: ...June 11 2015 715004752 Rev A Page viii...
Page 14: ...June 11 2015 715004752 Rev A Page xiv...
Page 46: ...June 11 2015 715004752 Rev A Page 46...
Page 124: ...June 11 2015 715004752 Rev A Page 124...
