June 11, 2015, 715004752 Rev. A
Page 95
•
flush buffered mobile phases out of the detector with HPLC-grade water followed
by a 5 to 10% methanol solution each time the detector is powered-off. This
process prevents,
–
plugging of the solvent lines and flow cell.
–
damage to the components.
–
microbial growth.
4.4 Maintaining the flow cell
Note:
To ensure proper initialization of the detector and the long serviceability of
flow cells, use well-degassed eluents, making sure they are flowing before you
power-on the detector.
A dirty flow cell can cause baseline noise, decreased sample energy levels, calibration
failure, and other problems with operation of the detector.
There are two phases in cleaning the flow cell:
•
Flushing
•
Removing and cleaning
If flushing is ineffective, remove and clean the flow cell. Replace flow cell components,
as required.
Note:
Always replace the flow cell gaskets when cleaning, rebuilding, or replacing
other flow cell components.
4.4.1 Flushing the flow cell
Flush the flow cell when it becomes contaminated with the residues of previous runs
and after each detector shutdown. A dirty flow cell can cause baseline noise,
decreased energy levels, calibration failure, and other problems. Always flush and
purge the flow cell as an initial attempt to correct these problems.
You should flush the flow cell whenever,
•
noise is higher than expected;
•
noise test results are not meeting specifications;
•
the detector fails to normalize.
If you use buffered mobile phase, flush it from the detector before powering-off.
Notice:
To avoid damaging the flow cell during reverse flushing, do not
overpressure the cell.
Summary of Contents for 2489
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