Waters 2489, Overview And Maintenance Manual

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June 11, 2015, 715004752 Rev. A
Page xiii
B Specifications ........................................................................ 137
B.1 Physical specifications ............................................................................. 137
B.2 Environmental specifications .................................................................... 137
B.3 Electrical specifications ............................................................................ 138
B.4 Performance specifications ....................................................................... 139
B.5 Optical component specifications .............................................................. 139
B.6 Flow cell specifications ............................................................................ 140
C Solvent Considerations .......................................................... 141
C.1 Introduction ........................................................................................... 141
C.1.1 Preventing contamination ............................................................... 141
C.1.2 Clean solvents .............................................................................. 141
C.1.3 Solvent quality.............................................................................. 141
C.1.4 Preparation checklist...................................................................... 141
C.1.5 Water .......................................................................................... 142
C.1.6 Using buffers ................................................................................ 142
C.1.7 Tetrahydrofuran ............................................................................ 142
C.2 Solvent miscibility .................................................................................. 142
C.2.1 How to use miscibility numbers ....................................................... 143
C.3 Buffered solvents ................................................................................... 144
C.4 Head height ........................................................................................... 144
C.5 Solvent viscosity .................................................................................... 144
C.6 Mobile phase solvent degassing ................................................................ 145
C.6.1 Gas solubility ................................................................................ 145
C.7 Solvent degassing methods ..................................................................... 146
C.7.1 Sparging ...................................................................................... 146
C.7.2 Vacuum degassing......................................................................... 146
C.7.3 Solvent degassing considerations .................................................... 146
C.8 Wavelength selection .............................................................................. 147
C.8.1 UV cutoffs for common solvents ...................................................... 147
C.8.2 Mixed mobile phases...................................................................... 148
C.8.3 Wavelength selection for chromophore detection ............................... 149