June 11, 2015, 715004752 Rev. A
Page 45
2.7.3 Assembling the fittings (HPLC only)
To assemble each fitting:
1. Slide the compression screw over the tubing end, followed by the ferrule.
2. Mount the ferrule with its tapered end facing the end of the tubing.
Figure 2–14: Ferrule and compression screw assembly
2.7.4 Making tubing connections in HPLC systems
To make tubing connections in an HPLC system:
1. Seat each tubing end in the column outlet, detector inlet, or detector outlet
fitting.
2. Seat each ferrule by tightening the compression screw 1/2 turn past
finger-tight.
Requirement:
To ensure accurate verification, power-on the detector before
pumping any mobile phase or solvent through the flow cell.
Recommendation:
To prevent reabsorption of dissolved oxygen (for systems using
vacuum degassers), run the solvent degasser continuously when operating the
detector at wavelengths less than 230 nm.
2.7.5 Making tubing connections in ACQUITY Arc systems
To make tubing connections in an ACQUITY Arc system:
1. Bottom each tubing end in the column outlet, detector inlet, or detector outlet
fitting.
2. Seat each ferrule by tightening the compression screw finger-tight.
Requirement:
To ensure accurate verification, power-on the detector before
pumping any mobile phase or solvent through the flow cell.
Recommendation:
To prevent reabsorption of dissolved oxygen (for systems using
vacuum degassers), run the solvent degasser continuously when operating the
detector at wavelengths less than 230 nm.
Compression
screw
Tube
Ferrule
Tubing end (cut
straight and smooth
to achieve maximum
column efficiency)
Distance (determined by each
application, such as union or
column fitting)
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