Waters 2489, Overview And Maintenance Manual

Page: 85 / 150

June 11, 2015, 715004752 Rev. A
Page 85
• AU1 – minimum absorbance displayed. The default is Auto.
• AU2 – maximum absorbance displayed. The default is Auto.
Using this function, you zoom in on various sections (artifacts) of the spectrum.
The scale of the spectrum is affected by the AUFS setting.
8. Press Next to advance through the four scaling parameters. The figure below
shows the sample scanned in the figure on page 84 . The sample has been
scaled by changing the wavelength parameters to 225 and 600 nm.
Figure 3–29: Sample erbium scan with
λ
1 changed to 225 nm and
λ
2
changed to 600 nm
9. Once you change one or more scaling parameters, press Enter to reformat the
graphical display.
10. Once the scan reappears, press Next to display the four highest peaks of the
scaled scan.
Figure 3–30: Four highest peaks from the scaled sample erbium scan
11. Press Next again to return to the sample scan display.
12. When you finish manipulating the sample scan graphical display, press SCAN
(Shift Chart-mark) to return to the Scan choice list.
Tip:
To store the scan, see “Storing a spectrum” on page 86 .
The figure below shows a series of scans of anthracene dissolved in acetonitrile to
display the use of the Scale function. The zero scan is not displayed.
For the scaling parameters AU1 and AU2, the default is Auto. You can change the AU
parameter based on the absorbance of the spectrum. To return the default to auto,
press CE.