Waters 2489, Overview And Maintenance Manual

Page: 148 / 150

June 11, 2015, 715004752 Rev. A
Page 148
C.8.2 Mixed mobile phases
The table below contains approximate wavelength cutoffs for some other solvents,
buffers, detergents, and mobile phases. The solvent concentrations represented are
those most commonly used. If you want to use a different concentration, you can
determine approximate absorbance using Beer’s Law, because absorbance is
proportional to concentration.
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene dichloride 230 Xylene 290
Table C–3: Wavelength cutoffs for different mobile phases:
Mobile phase
UV
cutoff
(nm)
Mobile phase
UV
cutoff
(nm)
Acetic acid, 1% 230 Sodium chloride, 1 M 207
Ammonium acetate,
10 mM
205 Sodium citrate, 10 mM 225
Ammonium bicarbonate,
10 mM
190 Sodium dodecyl sulfate 190
BRIJ 35, 0.1% 190 Sodium formate, 10 mM 200
CHAPS, 0.1% 215 Triethyl amine, 1% 235
Diammonium phosphate,
50 mM
205 Trifluoracetic acid, 0.1% 190
EDTA, disodium, 1 mM 190 TRIS HCl, 20 mM, pH 7.0,
pH 8.0
202, 212
HEPES, 10 mM, pH 7.6 225 Triton-X™ 100, 0.1% 240
Hydrochloric acid, 0.1% 190
Waters PIC
®
Reagent A,
1 vial/liter
200
MES, 10 mM, pH 6.0 215 Waters PIC Reagent B-6,
1 vial/liter
225
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
190
190
Waters PIC Reagent B-6,
low UV, 1 vial/liter
190
Sodium acetate, 10 mM 205 Waters PIC Reagent D-4,
1 vial/liter
190
Table C–2: UV cutoff wavelengths for common chromatographic solvents:
Solvent
UV cutoff
(nm)
Solvent
UV cutoff
(nm)