June 11, 2015, 715004752 Rev. A
Page 69
9. Record the numbers for later comparison.
Rule:
Run this procedure each time you change the detector lamp.
10. Flush the flow cell with approximately 30 to 60 mL of HPLC-grade methanol at
1 mL/min for 15 minutes, minimum, to clean the cell.
Make the plumbing connections described in
“Plumbing the detector” on page 43
before beginning the peak response test.
3.2.8 Wavelength calibration
You can calibrate the detector, manually, from the keypad at any time during
operation or if calibration errors arise during startup.
To calibrate the detector manually:
1. Press Calibrate (Shift 3) from the detector keypad.
Result:
A message appears asking whether you removed the cuvette and
flushed the flow cell with a transparent solvent (Waters recommends methanol
or water).
2. Press Enter, to continue the calibration cycle, or Cancel, to invoke the
absorbance screen without calibrating the detector.
Result:
After you press Enter, the detector cycles through the calibration
procedure and briefly displays a series of initialization messages similar to those
you saw at startup (see
).
If calibration is successful, the detector beeps three times and displays the
maximum error, in nanometers, of the calibration shift farthest from the last
calibration.
3. Press Enter to complete the calibration.
Result:
A “Calibration complete” message appears momentarily. Other
messages such as “Optimizing system performance” and “Restoring last setup”
can appear before the display returns to the absorbance screen.
If calibration is successful, the error message (<Error>) that was displayed on
the absorbance screen before you recalibrated the detector disappears.
4. If calibration is unsuccessful, retry, cycle power to the detector, or see
3.2.9 Operating the detector in single-wavelength mode
The detector is optimized for single-wavelength operation, the default operating
mode.
To invoke single-wavelength mode:
1. If the detector is in dual-wavelength (the wavelength icon shows
λλ)
mode, from
the absorbance (or HOME) screen, press the
λ/λλ
key (Shift Auto Zero).
Result:
The detector displays a message indicating it is switching to
single-wavelength operation.
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