Waters 2489, Overview And Maintenance Manual

Page: 96 / 150

June 11, 2015, 715004752 Rev. A
Page 96
Requirement:
Always use well-degassed eluents.
To flush the flow cell:
1. Stop the solvent flow, and remove the column.
2. Replace the column with a union or piece of tubing.
Note:
If the mobile phase is not compatible with water, flush with an
intermediate solvent first.
3. Flush the detector with HPLC-quality water.
4. Pump 100% methanol through the flow cell to clean it internally. Do not exceed
1000 psi.
5. Pump a strong cleaning solvent, such as isopropanol, through the flow cell
(optional). Do not exceed 1000 psi.
Note:
If the mobile phase is not miscible in water, first use an intermediary
solvent.
6. Resume pumping mobile phase.
7. Reattach the column.
8. If the flow cell remains dirty or blocked, reverse flush it.
To perform optimal cleaning:
1. Extinguish the detector lamp.
2. Stop the solvent flow, and then disconnect the column inlet and flow cell inlet
tubing.
Tip: For more efficient flushing, replace the column with a union or piece of
tubing.
3. Connect the column inlet tubing to the flow cell inlet.
4. If another instrument is downstream of the flow cell outlet, break the
connection at the other instrument, and route the outlet tubing to waste while
flushing.
5. Flush the detector using a miscible solvent and HPLC-quality water at the
recommended flow rate.
6. Flush the detector using HPLC-quality water at the recommended flow rate. If
the mobile phase is not compatible with water, flush using an intermediate
solvent first.
7. Pump 100% acetonitrile through the flow cell at the recommended flow rate to
clean it internally.
Notice:
If the flow cell will not be used for several days, flush it with clean
mobile phase, such as a water/acetonitrile or water/methanol, and then cap the
flow ports or dry the flow cell with pure nitrogen or pure helium.
Notice:
To prevent flow cell failure, do not connect any tubing or device that
can create backpressure exceeding the flow cell’s maximum rating of 1000 psi.