Waters 2489, Overview And Maintenance Manual

Page: 103 / 150

June 11, 2015, 715004752 Rev. A
Page 103
4.4.3.6 Replacing the flow cell
The detector is shipped with a standard analytical flow cell installed. Replace the flow
cell when
• the flow cell becomes damaged;
• you want to use one of the optional flow cells (see the table on page 140 ).
To prepare for replacing the flow cell:
1. Flush columns using at least 10 column-volumes of clean mobile phase before
connecting them to the flow cell.
Example:
flush a 4.6 × 50 mm HPLC column for 10 minutes at the
recommended flow rate.
2. Unpack and inspect the new flow cell.
3. Power-off the detector and disconnect the power cord.
4. Open the front door.
5. Disconnect the detector’s inlet and outlet tubing from the main column
connection and cap.
To replace the flow cell:
1. Using a 1/4-inch flat-blade screwdriver, loosen the three captive screws on the
front plate of the flow cell assembly.
2. Gently pull the assembly toward you.
3. Insert the new flow cell assembly into the detector.
4. Tighten the captive screws.
5. Reconnect the inlet/outlet tubing to your LC system.
6. Reconnect the power cord, and then power-on the detector.
4.5 Replacing the lamp
Recommendation:
Waters recommends that you change the detector lamp when
it repeatedly fails to ignite, or when the detector fails to calibrate.
Recommendation:
Invoke the lamp optimization software routine at least once a
week by powering-off the detector, waiting 10 seconds, and then powering-on the
detector.
See also:
Online Help, for alarm messages and recommended actions you can take
to correct them and “Using the diagnostic tests” on page 115 .
Notice:
To prevent contamination, wear clean, chemical-resistant, powder-free
gloves when disassembling, inspecting, cleaning, or replacing parts within the
Waters patented TaperSlit Flow Cell or when removing or replacing the flow cell
within its assembly.