June 11, 2015, 715004752 Rev. A
Page 70
2. Enter the wavelength and sensitivity on the absorbance screen, as well as any
secondary parameters and timed or threshold events.
Note:
Changing the sensitivity (AUFS) setting affects the 2-V output.
3. Select a second sensitivity setting while in single-wavelength mode, by pressing
A/B and specifying the appropriate AUFS values on the channel B screen.
Result:
A single-wavelength is tracked on channel A so that you can use
channel B to monitor absorbance with an alternate AUFS setting, or to make the
primary absorbance measurement using a specific AUFS value on channel A.
Example:
While operating in single-wavelength mode, specifying an AUFS
value of 2.0000 on the second channel provides 1.000 V/AU on the channel B,
2-V output.
The detector automatically engages the second-order filter for all wavelengths
greater than 370 nm.
3.2.10 Operating the detector in dual-wavelength mode
You can operate the detector with expanded chart-out selections using
dual-wavelength mode. In addition to absorbance offered with single-wavelength
mode, dual-wavelength mode offers the following functions:
•
Absorbance (A and B)
•
MaxPlot
•
RatioPlot (A/B)
•
Difference plot (A-B)
To change from single to dual-wavelength mode:
1. From the absorbance (or HOME) screen, when the detector is in
single-wavelength mode (the wavelength icon shows
λ
), press the
λ/λλ
key
(Shift Auto Zero).
Result:
A momentary message “Setting up dual-wavelength mode” appears.
2. Enter the wavelength to be monitored in the
λ
field, and then press Enter.
3. Enter the other operating parameters and timed or threshold events, if desired.
4. Press the A/B key to switch channels.
Result:
The absorbance screen for the other channel appears.
5. Enter the operating parameters for monitoring the second wavelength, including
timed and threshold events, if desired.
Tips:
•
If both selected wavelengths are greater than 370 nm (+/– 1 nm), the detector
applies the second-order filter to block unwanted UV light.
•
If both selected wavelengths are less than 370 nm (+/– 1 nm), the detector
removes the second-order filter.
•
If the selected wavelengths bracket the 370 nm (+/– 1 nm) threshold, the
detector does not apply the second-order filter and issues a warning message
stating that any data collected for the wavelength above 370 nm can contain
inaccuracies because of possible UV light interference (second-order effects).
Summary of Contents for 2489
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