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PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
Assays/Reagents
AEROSET
®
System Troubleshooting Guide
99
94816-107—November 2004
Limit of Quantitation for Abbott Assays Data Sheet Example
Date: 3/11/03 Analyte: ABC
Instrument: AEROSET System Serial No.: 1243
Record the values for the low analyte sample replicates.
Calculate the limit of quantitation.
Comments: Confirmed limit of quantitation
_______________________________________________________________________________________
Technologist: (Name) Date: 3/11/03
Reviewed by: (Name) Date: 3/11/03
Lot Number
Expiration Date
Reagent Kit
334455MB
07/07/03
Calibrators
50383
05/01/03
Replicate
Value
1
0.25
2
0.25
3
0.25
4
0.23
5
0.20
6
0.26
7
0.24
8
0.35
9
0.35
10
0.25
A. Determine the mean of the replicates:
Mean = 0.26
B. Determine the SD of the replicates:
SD = 0.0481
C. Determine the %CV of the sample:
%CV = 18.5
Data Summary
Calculated Value
Expected Value
Limit of Quantitation
18.5%
20.0%
SD
Mean
× 100
%CV =