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PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Not for Customer Distribution
Assays/Reagents
AEROSET
®
System Troubleshooting Guide
141
94816-107—November 2004
Frequently Asked Questions (FAQ)
Question
Response
MULTIGENT
®
Hemoglobin A
1c
results do not
match another method. Why?
MULTIGENT Hemoglobin A
1c
is an indirect
method, in which lower HbA
1c
concentrations produce higher signal levels.
This may provide less imprecision at these
lower concentrations. The reading
wavelength of 604 nm minimizes interference
from HAMA (human anti-mouse antibodies),
hemolysis, bilirubin, and lipemia.
Which controls are recommended? Which
controls have AEROSET assay values?
MULTIGENT Hemoglobin A
1c
controls,
LN 2K96-10, are recommended for the
Hemoglobin A
1c
assay. Refer to the
MULTIGENT Hemoglobin A
1c
Control value
sheet for control ranges.
Why am I getting negative calibration rates?
Is this normal?
This is normal. The indirect assay method
provides a curve in which the signal is
inversely proportional to concentration,
i.e.
,
low sample values produce the highest rates.
The zero calibrator rate (highest rate) is used
as a blank and is subtracted from the rates of
all the other calibrators, causing them to be
negative.
How does this assay perform with specific
hemoglobin variants,
e.g.
, HbA
2
, HbF, HbS?
Refer to the Interfering Substances section of
the Hemoglobin A
1c
package insert.
The Reaction Mode for Total Hemoglobin is
listed as END UP in the package insert. Why
does the Reaction Graph appear to show an
END DOWN reaction?
• This occurs because sample and reagent are
added and mixed at Read Time 1. However,
it takes time for the reaction mixture to
settle. Bubbles and swirling of the mixture
in the cuvette cause absorbance of the
sample to start at a higher number than
when the final read is taken.
• The reaction is classified as END UP because
absorbance increases with increasing
analyte concentration within the main read
window.