PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Assays/Reagents
Not for Customer Distribution
102
AEROSET
®
System Troubleshooting Guide
94816-107—November 2004
Although Paramax and AEROSET assays definitely correlate (as
shown by the r value), a specimen tested on the AEROSET will
yield a value approximately three times greater than that
obtained by the Paramax System. The Paramax uses
alpha-naphthylphosphatase as the substrate for the ACP
reaction, as does the AEROSET and Vitros (Array substrate
unavailable). Despite the use of the same substrate and
generally similar reaction schemes, different reagents do not
yield similar results. The Paramax employs a solid tablet
reagent format, the Vitros a unique dry (damp) slide format,
and the Array and AEROSET both use liquid reagents. The
exact content of all of these reagents is not known, this is
proprietary information. Given the many factors affecting ACP
analysis, such as heterogeneity of the enzyme (different
isozymes are found in liver, spleen, erythrocytes, prostate,
etc.), differences in conditions for optimal activity of these
isozymes (pH, inhibitors, etc.), need to maintain an acidic
environment to stabilize and preserve ACP activity (addition
of an acidifying solution to serum specimens after collection),
and the variety of different reagent formulations that exists, it
is not surprising that roughly equivalent results are not
obtained when the same specimens are tested using kits from
different vendors.