PROPRIETARY INFORMATION
CONFIDENTIAL MATERIAL
Flowcharts
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248
AEROSET
®
System Troubleshooting Guide
94816-107—November 2004
Abbott Assay—Basic Assay Troubleshooting, Continued
Assay Characteristics
If multiple assays are affected, look for similar characteristics
such as:
•
Same line
•
Assays using R1 only vs. assays using R1 and R2
•
Similar wavelengths
•
Same calibrators
•
Same sample and reagent volumes
•
Rate vs. end-point reaction
If the issue affects one assay:
•
Verify assay parameters with the assay-specific package
insert
•
Was a different bottle of reagent, calibrator, or control
used, and was it handled correctly?
•
Was a different lot of calibrator or reagent used?
•
Are there any non-Abbott assays on the original line? If so,
were carryover studies performed between those assays
and the affected assay?
•
Move the assay to an alternate line. If the issue is not
resolved, proceed to step 2 of
Instrument
Troubleshooting
.
Instrument Troubleshooting
1. Is the issue on both lines? If yes, troubleshoot common
items, including:
•
Lamp
•
Cuvette washer
•
Water
•
Bulk solution use
•
Maintenance
•
Probe/mixer wash flow