horiba FluoroMax-3, Руководство по эксплуатации

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FluoroMax-3 v. 3.1 (6 Mar 2006) Glossary
13-2
Bandpass filter
An optical element, which selectively transmits a range of wave-
lengths, while absorbing light of higher and lower wavelengths.
Bioluminescence
Emission of light originating from a chemical reaction in a living or-
ganism.
Blank subtraction
The removal of the spectral response of the solvent (and sample con-
tainer) from the sample’s spectral response. To accomplish this, an
identical scan is run on the solvent just before running the actual
sample. Proper use of a blank can remove solvent luminscence arti-
facts, scattering events, and any artifacts from the sample cuvette or
container. In the
Experiment Setup
window, under the
Detectors
icon, check the
Blank Subtract
checkbox to acquire a blank.
Blaze wavelength (of grat-
ings)
The wavelength at which a grating is optimized for peak efficiency.
As a rough guide, gratings are usable from 2/3 of the blaze wave-
length to twice the blaze wavelength. The excitation and emission
gratings for the FluoroMax
®
-3 are blazed for efficiency in the UV
and visible.
Chemiluminescence
Emission of light originating from a chemical reaction.
Concentration determina-
tion
A function of the
Single Point
type of scan, that calculates an un-
known sample’s concentration. The user runs known samples and
enters the concentration in order to calibrate the routine. Then an as-
say may be completed with the measurements based on concentra-
tion.
Corrected emission scan
An emission scan that has been corrected for the wavelength re-
sponse of the emission monochromator and the signal detector. To
obtain a corrected emission scan, an emission spectrum is multiplied
by the appropriate emission correction-factor file. A set of emission
correction factors is supplied with the instrument and stored under
the name
MCORRECT
.
SPC
.
Corrected excitation scan
An excitation scan corrected for the wavelength-characteristics of the
xenon lamp, the aging of the xenon lamp, and the gratings in the ex-
citation spectrometer. To obtain a first-order correction of the excita-
tion scan, the emission detector signal is ratioed to the reference sig-
nal (i.e.,
S/R ). This provides correction for the lamp and excitation-
monochromator spectral response, which is ~95% of the required
correction. To obtain a completely correct scan, the excitation scan
acquired in the
S/R acquisition mode is multiplied by excitation cor-
rection factors. A set of excitation correction factors (
XCORRECT
.
SPC
)
is included.
Correction factors
A spectral set of multiplicative factors used to compensate for the
instrumental wavelength-response from detectors and optics. These
correction factors are specific to a particular optical configuration for
the instrument, and must be run for each detector in the spectro-
fluorometer system. Excitation and emission correction factors are
included by default with each new spectrofluorometer. Their default