horiba FluoroMax-3, Руководство по эксплуатации

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FluoroMax-3 v. 3.1 (6 Mar 2006) Automated Polarizers
10-14
Using automated polarizers
FluorEssence™ software with Spex
®
polarizers provides many choices for polarization
measurements. Depending on the accessories, the opportunity exists to remove polari-
zation effects from the sample, measure the polarization characteristics, or analyze the
decay of anisotropy using frequency-domain techniques. For further software informa-
tion, refer to the FluorEssence™ and Origin
®
on-line help.
Applications for polarizers
•
Measurement of emission anisotropy or polarization at fixed wavelengths. This is
used for binding assays, kinetics of molecular size or shape change, temperature ef-
fects on rotational motion of fluorophores (e.g., phase transition of phospholipid bi-
layers).
•
Measurement of excitation and emission spectra using magic angles. This helps to
eliminate spectral artifacts.
•
Measurement of a principal polarization or excitation anisotropy spectrum, using an
excitation scan with polarization or the
POLAR.AB
macro acquisition. This pro-
vides information about rotational sensitivity of the excitation spectrum by measur-
ing <
r > versus λ
exc
(with λ
em
constant). Examine relative molecular dipole-angles at
cryogenic temperatures in a viscous solvent.