horiba FluoroMax-3, Руководство по эксплуатации

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FluoroMax-3 v. 3.1 (6 Mar 2006) FluoroMax
®
-P Phosphorimeter Operation
9-6
Isolate components in a mixture based on lifetimes
To the right are three scans
of an aqueous mixture of
terbium and europium chlo-
rides that isolate different
phosphorescent compo-
nents based on their life-
times. For example, a sam-
ple containing two phos-
phorescent species with dif-
ferent lifetimes (Tb = 421
µs; Eu = 113 µs) can be re-
solved into its components
by varying the
Initial Delay
parameter, here shown as
bold italic numerals,
0.1
ms,
1
ms, and
2
ms initial
delay. Notice the wave-
length shift in the 593 nm
peak (marked with a gray
line) from initially mostly
europium fluorescence (short lifetime) to a mixture of terbium (longer lifetime) and
europium fluorescence at later delays. Also notice the Eu peak near 690 nm at 0.1 ms
that vanishes at later times. This experiment was performed using the
Emission
sub-
type of
Phos
experiment.
Three-dimensional plots
Using the phosphorescence delay curve with the
Initial Delay
technique, a three-
dimensional graph can be created, as shown below. This graph shows successive scans
of a mixture of Tb-L and Eu-L (where L = benzophenone antenna chromophore) with
varying time delays, segregating species into contours representing a particular slice of
time.