horiba FluoroMax-3, Руководство по эксплуатации

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FluoroMax-3 v. 3.1 (3 Mar 2006) Data Acquisition
4-12
Note:
To minimize Rayleigh scatter, offset the start position by at
least 15 nm from the excitation wavelength, with a bandpass of
5 nm. For example, for an excitation wavelength of 300 nm, use
315 nm as the start. Set the ending wavelength to 550 nm. Use
an increment of 2 nm and an integration time of 0.1 s.
crement of 1.0 nm, an integration time of 0.1–0.5 s, and one scan. If unsure of
an excitation wavelength, try 300 nm, at which many samples absorb light. Use
S (signal detector) for the acquisition mode. Don’t forget a data file name.
j
Insert the sample into the sample compartment, and close the sample-
compartment’s cover.
k
Click
Run
.
The scan starts.
l
With the spectrum on the screen, note the greatest intensity.
If the signal exceeds 12 µA, then the emission detector is saturating, so close the
slits more. If there is no obvious peak, increase the excitation wavelength, start-
ing, and ending by 25 nm, and retry a scan.
m
With an acceptable emission peak, record its wavelength.
This is the emission maximum.
Otherwise, repeat steps
i through l until an obvious emission peak appears.
2
Find the optimal excitation wavelength.
This procedure uses the emission maximum to determine the optimum excita-
tion wavelength, and is similar to step 1.
a
Click the
Experiment Menu
button:
The
Fluorescence Main Experiment Menu
appears: