horiba FluoroMax-3, Руководство по эксплуатации

Страница: 62 / 220

FluoroMax-3 v. 3.1 (3 Mar 2006) Data Acquisition
4-10
Running an unknown sample
Often a researcher will scan a sample whose spectral characteristics are unknown. For
optimal spectra, the optimal excitation and emission wavelengths must be found.
The optimal excitation wavelength is the wavelength that creates the most intense emis-
sion spectrum for a given sample. For many samples, the optimum wavelengths are
known. For a sample whose wavelength positions are unknown, the user must deter-
mine these wavelengths to obtain the best possible results.
The traditional method consists of running an emission scan to find the peak emission
value. Then an excitation scan is run using the determined peak emission value.
1
Find the preliminary emission maximum.
The object of this step is to acquire a preliminary emission scan, based on a
“best-guess” excitation wavelength. Because the fluorescence emission of sam-
ples does not shift with excitation wavelength, the guessed excitation wave-
length yields the emission peak, albeit perhaps at lower intensity.
a
Be sure all system components are on, and the FluoroMax
®
-3 is cali-
brated as explained in Chapter 3.
b
Set up the sample with the fiber-optic probe, with minimal stray-light in-
terference.
c
Click the Experiment Menu button:
The
Fluorescence Main
Experiment Menu
appears:
d
Choose
Spectra
.
The
Experiment Type
menu appears.