3
OMNICxi Software Operation
Collect a Mosaic
122
DXRxi User Guide
Thermo Scientific
10. Click
FOCUS Video
in the software to bring the sample into sharp focus (adjust
brightness as needed).
The sample should still be centered under the new objective and focused in the left
(video) pane.
11. Click
Zoom To Region
in the software to center and scale the optical image (right
pane).
12. Click
START Mosaic
to collect a mosaic.
The microscope stage begins moving the sample in a spiral pattern while the camera takes
static pictures of the sample surface so you can view a larger area in the software. The
pictures appear in the right (mosaic) pane (see
).
The pictures are stitched together with the edges slightly overlapped to ensure that all
areas are captured. The software continues collecting pictures until the mosaic pane (at
the current zoom level) is filled or until you click STOP Mosaic in the software. We refer
to the combined pictures as a mosaic.
To collect a terrain mosaic
1.
onto the microscope stage.
2.
.
3. Click the
Video
button in the software to select video mode.
4. Open the sample compartment doors on the microscope and rotate the desired objective
to the front objective position (for easier initial focusing, start with the 10x objective).
5. Select the video calibration for this objective by setting the
Objective
option in the
OMNICxi Acquisition window.
6. Set the
on the microscope to its middle position to direct light through
the microscope eyepiece and to the video camera.
7. Use the microscope to locate and bring the sample into focus.
Note
If your sample is large, you can collect a mosaic before you switch to the higher
magnification objective. Collecting the mosaic with a low magnification objective is
faster and uses less computer memory. However, the resulting mosaic may not show
sufficient detail if the sample or features of interest are very small.
Note
If the objective you selected on the microscope isn’t listed under the Objective
option in the software,
for that objective.
NOTICE
Be careful not to bump the sample into the lens when bringing the sample
into focus.
Summary of Contents for DXR xi
Page 12: ...1 Introducing the DXRxi Raman Imaging System Trademarks 6 DXRxi User Guide Thermo Scientific...
Page 14: ...2 DXRxi Microscope Operation 8 DXRxi User Guide Thermo Scientific...
Page 64: ...Safety Information 58 DXRxi User Guide Thermo Scientific Microscope Safety Labels or...
Page 65: ...Safety Information Thermo Scientific DXRxi User Guide 59...
Page 66: ...Safety Information 60 DXRxi User Guide Thermo Scientific...
Page 67: ...Safety Information Thermo Scientific DXRxi User Guide 61...
Page 68: ...Safety Information 62 DXRxi User Guide Thermo Scientific...
Page 166: ...3 OMNICxi Software Operation Acquire and Analyze Data 160 DXRxi User Guide Thermo Scientific...