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3
OMNICxi Software Operation
Acquire and Analyze Data
130
DXRxi User Guide
Thermo Scientific
Acquire and Analyze a Chemical Image
Create a chemical image to identify components of your sample or to gain understanding of
the sample’s structure and composition.
To acquire and analyze image data
1.
onto the microscope stage.
2.
.
3. Click the
Video
button in the software to select video mode.
4.
Bring the sample into focus and collect a mosaic
5. Use
Live spectrum mode
to acquire spectra at several locations of the sample in order to
optimize the
settings.
Table 24.
Acquisition window, Advanced settings for acquiring a chemical image
Feature
Description
Photobleach time
Time for each sample point to be exposed to the laser for
photobleaching before collection begins. Adding photobleach
time may reduce sample fluorescence but will also increase scan
time.
EM gain
Controls the electron multiplying gain of the detector camera.
EM gain increases the signal to overcome the readout noise, but it
also increases other sources of noise. The optimal setting for this is
highly dependent on the experiment. In general, it is
recommended to run with EM gain on for most operation but to
turn it off when collecting with a longer exposure time.
Laser polarization
(requires polarization
option)
Sets the polarization for the laser light incident on the sample
Analyzer polarization
(requires polarization
option)
Sets the position for the polarization analyzer controlling the
Raman emission. This option is only available when Laser
polarization is set to "Horizontal(X)" or "Vertical(Y)" to run a
polarized experiment.
Analyzer angle
(requires polarization
option)
Sets an arbitrary angle (0–180 degrees) for the polarization
analyzer controlling the Raman emission. This option is only
available when Analyzer angle is set to "Select Angle."
Summary of Contents for DXR xi
Page 12: ...1 Introducing the DXRxi Raman Imaging System Trademarks 6 DXRxi User Guide Thermo Scientific...
Page 14: ...2 DXRxi Microscope Operation 8 DXRxi User Guide Thermo Scientific...
Page 64: ...Safety Information 58 DXRxi User Guide Thermo Scientific Microscope Safety Labels or...
Page 65: ...Safety Information Thermo Scientific DXRxi User Guide 59...
Page 66: ...Safety Information 60 DXRxi User Guide Thermo Scientific...
Page 67: ...Safety Information Thermo Scientific DXRxi User Guide 61...
Page 68: ...Safety Information 62 DXRxi User Guide Thermo Scientific...
Page 166: ...3 OMNICxi Software Operation Acquire and Analyze Data 160 DXRxi User Guide Thermo Scientific...