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CSB CryoEM Facility TF20 Operating Instructions SEC
05/02/16
The Tecnai F20 cryo electron microscope is a high resolution electron microscope that can be operated at
120 or 200kV. This microscope is equipped with a Field Emission Gun (FEG) as its illumination source and is
suitable for data collection of negative stained as well as cryo preserved samples. Three different types of
holders are available for use: a room temperature (RT) for setup and 0degree tilt data collection, a Fischione
RT holder for collecting random conical tilt negativestained images, and two cryo TEM holders for vitrified
samples (Gatan 626). The TF20 is equipped with a 4k by 4k Gatan UltraScan CCD camera for digital image
collection. The compustage of the microscope allows for precise movements and tilts to up to +/ 70 degrees.
The microscope’s main parts are shown below: