Glossary of Terms
171
P/N CLS151164 Rev. B
LabChip GX Touch/GXII Touch User Manual
PerkinElmer, Inc.
Hardware Diagnostics
Whenever a run begins, the instrument checks for errors (
e.g.,
defective high-voltage supplies, missing conductivity between wells,
etc.). If an
error
is detected, a message box displays and the run
stops. The message box describes the problem and either contains
information on how to resolve the problem or directs you to call
PerkinElmer Technical Support (see
)
.
Inflection Threshold
Peaks that are very close together are identified as a single peak if
the peaks do not have a clear valley between them. The Inflection
Threshold property splits peaks based on the slope. The inflection
threshold defines the value that the slope minimum must be below
to trigger a splitting of the peak. As the threshold is increased, more
peak splitting occurs. Analysis settings can only be changed when
reviewing LabChip GX Touch data in the LabChip GX Reviewer
software.
Lab-on-a-Chip
The generic term for a microfluidic product, signifying a chemical
process or material movement taking place on a microchip. In
contrast to analysis in a standard laboratory that relies on human
intervention at several stages to manipulate or observe samples
and record results, the self-contained lab-on-a-chip represents an
almost hands-free technology.
Ladder
Ladders are used to align the data and calculate the size of data
peaks. (Protein Charge Variant assays do not use ladders or
markers.) The Ladder tube is located in the back right corner of the
microplate carrier, next to the buffer tube. The Ladder is sipped
before the first well and then after every 12 wells. The ladder is
analyzed before sample analysis starts.
The peak sizes and markers defined for the ladder in the assay are
assigned consecutively, starting with the first peak detected in the
ladder. If too few peaks are detected in the ladder well, analysis
stops and an error is generated. Peaks appearing above the upper
marker do not have to be detected.
