Glossary of Terms
177
P/N CLS151164 Rev. B
LabChip GX Touch/GXII Touch User Manual
PerkinElmer, Inc.
For the purpose of quantitizing the full RNA content in the sample, a
straight-line baseline is drawn across the bottom of the sample by
finding the local averages about its endpoint. Trapezoidal
integration from the straight-line baseline to the data signal is used
to calculate the total RNA area. Integration is performed from the
end of the lower marker to the endpoint of the area baseline. The
range from the end of the 5S to the start of the 18S peak is termed
the Fast Area. This area is calculated in the same way as the total
RNA area.
For the purpose of quantitizing the rRNA peaks, a two-point
baseline is drawn across the bottom of each peak identified as
rRNA and the area is computed. These areas and rRNA peak
heights and some relevant ratios are exported in the Well Table. A
combination of the quantities is used to assess the quality of the
RNA sample. Analysis settings can only be changed when
reviewing LabChip GX Touch data in the LabChip GX Reviewer
software.
Sample Names File
Sample Name are used to import sample names into a data file as
the data file is created. The Sample Names File can also contain
expected peaks. The sample names file is c
reated in the LabChip
GX Reviewer or LabChip GX Touch software.
See the
LabChip GX
Reviewer User Manual
for details on creating the sample names file
in LabChip GX Reviewer. Sample Name that contain 96 wells or
384 wells can be created in the LabChip GX Touch software (see
Start Time
This setting determines the time after the start of a run when peaks
will be detected. Any peaks appearing before this time are ignored.
Analysis settings can only be changed when reviewing LabChip GX
Touch data in the LabChip GX Reviewer software.
